Midkine (MK) ELISA Kit for Rat

Name: Rat MK ELISA Kit; Midkine; MyBioSource
Brand: MyBioSource
SKU: MBS2702184
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2702184

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Midkine (MK). Standards or samples are then added to the appropriate microtiter plate wells with… More details

$295 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Midkine (MK), ELISA Kit

Full Product Name

Rat Midkine (MK) ELISA Kit

Product Gene Name

MK elisa kit

Product Synonym Names

MDK; ARAP; NEGF2; Neurite Growth-Promoting Factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2

Species Reactivity

Rat

Assay Type

Double-antibody Sandwich

Samples

Serum, Plasma and Other Biological Fluids

Detection Range

15.62-1000pg/mL

Sensitivity

< 7.2pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Midkine (MK).
No significant cross-reactivity or interference between Midkine (MK) and analogues was observed.

Preparation and Storage

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Molecular Weight

9,512 Da

OMIM

M69148 mRNA

3D Structure

ModBase 3D Structure for P21741

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Intra-assay Precision (Precision within an assay)

3 samples with low, middle and high level Midkine (MK) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays)

3 samples with low, middle and high level Midkine (MK) were tested on 3 different plates, 8 replicates in each plate.

CV(%) =

SD/meanX100

Intra-Assay

CV<10%

Inter-Assay

CV<12%

ISO Certification

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Other Notes

Small volumes of MK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MK purchase

MBS2702184 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Midkine (MK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MK. The ELISA analytical biochemical technique of the MBS2702184 kit is based on MK antibody-MK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MK. It may not necessarily be applicable to this product.

NCBI GI #

396080281

NCBI GeneID

4192

NCBI Accession #

NP_001257480.1

UniProt Primary Accession #

P21741

UniProt Secondary Accession #

Q2LEK4; Q9UCC7

UniProt Related Accession #

P21741

GenBank Nucleotide #

NM_001270551.1

NCBI Official Full Name

midkine isoform a

NCBI Official Synonym Full Names

midkine

NCBI Official Symbol

MDK

NCBI Official Synonym Symbols

MK; ARAP; NEGF2

UniProt Protein Name

Midkine

UniProt Synonym Protein Names

Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein

Protein Family

Midkine

UniProt Gene Name

MDK

UniProt Synonym Gene Names

MK1; NEGF2; MK; ARAP

NCBI Protein Information

midkine

NCBI Summary for MK

This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]

UniProt Comments for MK

Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development ().

Research Articles on MK

1. According to our results, serum MK has greater diagnostic value in diagnosing cancer, however, more reliable studies in larger cohort should be conducted to evaluate the diagnostic accuracy of serum MK.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MK elisa kit

Reference Product	PubMed Publications
ACE elisa kit	>2 publications with MK and ACE

Pathways associated with MK elisa kit

Products by Pathway	Pathway Diagram
Glypican 2 Network Pathway antibodies	Glypican 2 Network Pathway Diagram
NOTCH2 Activation And Transmission Of Signal To The Nucleus Pathway antibodies	NOTCH2 Activation And Transmission Of Signal To The Nucleus Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By NOTCH Pathway antibodies	Signaling By NOTCH Pathway Diagram
Signaling By NOTCH2 Pathway antibodies	Signaling By NOTCH2 Pathway Diagram
Syndecan-4-mediated Signaling Events Pathway antibodies	Syndecan-4-mediated Signaling Events Pathway Diagram

Diseases associated with MK elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>62 publications with MK and Neoplasms
Kidney Diseases Antibodies	>23 publications with MK and Kidney Diseases
Nervous System Diseases Antibodies	>17 publications with MK and Nervous System Diseases
Adenocarcinoma Antibodies	>13 publications with MK and Adenocarcinoma
Brain Diseases Antibodies	>11 publications with MK and Brain Diseases
Congenital Abnormalities Antibodies	>11 publications with MK and Congenital Abnormalities
Inflammation Antibodies	>9 publications with MK and Inflammation
Skin Diseases Antibodies	>9 publications with MK and Skin Diseases
Pain Antibodies	>6 publications with MK and Pain
Lung Neoplasms Antibodies	>6 publications with MK and Lung Neoplasms

Organs/Tissues associated with MK elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>39 publications with MK and Blood
Kidney Antibodies	>25 publications with MK and Kidney
Brain Antibodies	>19 publications with MK and Brain
Lung Antibodies	>13 publications with MK and Lung
Bone Antibodies	>13 publications with MK and Bone
Embryonic Tissue Antibodies	>10 publications with MK and Embryonic Tissue
Muscle Antibodies	>9 publications with MK and Muscle
Liver Antibodies	>8 publications with MK and Liver
Skin Antibodies	>6 publications with MK and Skin
Lymph Node Antibodies	>5 publications with MK and Lymph Node