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ABCC2 siRNA – Canalicular Multispecific Organic Anion Transporter 1 (Rat)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8223977

MBS8223977 is an siRNA targeting ABCC2. Also known as ABCC2 siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit ABCC2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ABCC2, siRNA
Full Product Name
ABCC2 siRNA (Rat)
Product Gene Name
ABCC2 sirna
Product Synonym Names
CMOAT; CMRP; MRP2; Canalicular multispecific organic anion transporter 1; ATP-binding cassette sub-family C member 2; Canalicular multidrug resistance protein; Multidrug resistance-associated protein 2
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
ABCC2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
173,384 Da
3D Structure
ModBase 3D Structure for Q63120
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ABCC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for ABCC2 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of ABCC2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ABCC2. It may not necessarily be applicable to this product.

NCBI GI #
6978669
NCBI GeneID
25303
NCBI Accession #
NP_036965.1
UniProt Primary Accession #
Q63120
UniProt Secondary Accession #
Q63145
UniProt Related Accession #
Q63120
GenBank Nucleotide #
NM_012833.2
NCBI Official Full Name
canalicular multispecific organic anion transporter 1
NCBI Official Synonym Full Names
ATP-binding cassette, subfamily C (CFTR/MRP), member 2
NCBI Official Symbol
Abcc2
NCBI Official Synonym Symbols
Mrp2; cMRP; Cmoat
UniProt Protein Name
Canalicular multispecific organic anion transporter 1
UniProt Synonym Protein Names
ATP-binding cassette sub-family C member 2; Canalicular multidrug resistance protein; Multidrug resistance-associated protein 2
Protein Family
ABC transporter C family
UniProt Gene Name
Abcc2
UniProt Synonym Gene Names
Cmoat; Cmrp; Mrp2
UniProt Entry Name
MRP2_RAT
NCBI Protein Information
canalicular multispecific organic anion transporter 1

NCBI Summary for ABCC2

multispecific organic anion transporter; mutation may be responsible for conjugated hyperbilirubinemia in the TR- rat, which is a model for human Dubin-Johnson syndrome [RGD, Feb 2006]

UniProt Comments for ABCC2

ABCC2: Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter. Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver. Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, ABC family

Cellular Component: intercellular canaliculus; cell surface; membrane; integral to plasma membrane; apical plasma membrane

Molecular Function: protein domain specific binding; protein binding; ATPase activity, coupled to transmembrane movement of substances; organic anion transmembrane transporter activity; ATP binding

Biological Process: response to drug; prostaglandin transport; response to estrogen stimulus; response to heat; metabolic process; response to steroid hormone stimulus; response to arsenic; multidrug transport; response to methotrexate; response to oxidative stress; cellular chloride ion homeostasis; organic anion transport
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Research Articles on ABCC2

1. hyperammonemia increases the function and expression of P-glycoprotein and multidrug resistance-associated protein 2 at the blood-brain barrier via activating NF-kappaB pathway

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ABCC2 sirna

 Reference Product  PubMed Publications
 Abcg2 sirna  >227 publications with ABCC2 and Abcg2
 Slco1b3 sirna  >34 publications with ABCC2 and Slco1b3
 Slc10a1 sirna  >16 publications with ABCC2 and Slc10a1
 Nr1i2 sirna  >13 publications with ABCC2 and Nr1i2
 Abcg8 sirna  >9 publications with ABCC2 and Abcg8
 Slco1a2 sirna  >9 publications with ABCC2 and Slco1a2
 Slc22a8 sirna  >8 publications with ABCC2 and Slc22a8
 Slco1a1 sirna  >4 publications with ABCC2 and Slco1a1
 Slco1c1 sirna  >2 publications with ABCC2 and Slco1c1
 Hnf4a sirna  >1 publications with ABCC2 and Hnf4a

Pathways associated with ABCC2 sirna

 Products by Pathway  Pathway Diagram
 ABC Transporters Pathway antibodies  ABC Transporters Pathway Diagram
 ABC Transporters Pathway antibodies  ABC Transporters Pathway Diagram
 ABC-family Proteins Mediated Transport Pathway antibodies  ABC-family Proteins Mediated Transport Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Irinotecan Pathway antibodies  Irinotecan Pathway Diagram
 Nuclear Receptors In Lipid Metabolism And Toxicity Pathway antibodies  Nuclear Receptors In Lipid Metabolism And Toxicity Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with ABCC2 sirna

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >84 publications with ABCC2 and Liver Diseases
 Cholestasis Antibodies  >71 publications with ABCC2 and Cholestasis
 Hyperbilirubinemia Antibodies  >57 publications with ABCC2 and Hyperbilirubinemia
 Nervous System Diseases Antibodies  >38 publications with ABCC2 and Nervous System Diseases
 Brain Diseases Antibodies  >34 publications with ABCC2 and Brain Diseases
 Drug-Induced Liver Injury Antibodies  >30 publications with ABCC2 and Drug-Induced Liver Injury
 Kidney Diseases Antibodies  >25 publications with ABCC2 and Kidney Diseases
 Breast Neoplasms Antibodies  >24 publications with ABCC2 and Breast Neoplasms
 Liver Neoplasms Antibodies  >24 publications with ABCC2 and Liver Neoplasms
 Drug-Related Side Effects and Adverse Reactions Antibodies  >22 publications with ABCC2 and Drug-Related Side Effects and Adverse Reactions

Organs/Tissues associated with ABCC2 sirna

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >400 publications with ABCC2 and Liver
 Kidney Antibodies  >170 publications with ABCC2 and Kidney
 Brain Antibodies  >59 publications with ABCC2 and Brain
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