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HSD17B4 siRNA – Peroxisomal Multifunctional Enzyme Type 2 (Rat)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8212366

MBS8212366 is an siRNA targeting HSD17B4. Also known as HSD17B4 siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit HSD17B4 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HSD17B4, siRNA
Full Product Name
HSD17B4 siRNA (Rat)
Product Gene Name
HSD17B4 sirna
Product Synonym Names
EDH17B4; Peroxisomal multifunctional enzyme type 2; MFE-2; 17-beta-hydroxysteroid dehydrogenase 4; 17-beta-HSD 4; D-bifunctional protein; DBP; Multifunctional protein 2; MPF-2
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
HSD17B4 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
79,428 Da
3D Structure
ModBase 3D Structure for P97852
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat HSD17B4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for HSD17B4 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of HSD17B4 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HSD17B4. It may not necessarily be applicable to this product.

NCBI GI #
162287198
NCBI GeneID
79244
NCBI Accession #
NP_077368.2
UniProt Primary Accession #
P97852
UniProt Secondary Accession #
P70523; P70540
UniProt Related Accession #
P97852
GenBank Nucleotide #
NM_024392.2
NCBI Official Full Name
peroxisomal multifunctional enzyme type 2
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 4
NCBI Official Symbol
Hsd17b4
NCBI Official Synonym Symbols
MPF-2
UniProt Protein Name
Peroxisomal multifunctional enzyme type 2
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase 4; 17-beta-HSD 4
UniProt Gene Name
Hsd17b4
UniProt Synonym Gene Names
Edh17b4; MFE-2; 17-beta-HSD 4; DBP; MPF-2
UniProt Entry Name
DHB4_RAT
NCBI Protein Information
peroxisomal multifunctional enzyme type 2

NCBI Summary for HSD17B4

a 17 beta-hydroxysteroid dehydrogenase type IV involved in cholesterol metabolism [RGD, Feb 2006]

UniProt Comments for HSD17B4

HSD17B4: Bifunctional enzyme acting on the peroxisomal beta- oxidation pathway for fatty acids. Catalyzes the formation of 3- ketoacyl-CoA intermediates from both straight-chain and 2-methyl- branched-chain fatty acids. Defects in HSD17B4 are a cause of D-bifunctional protein deficiency (DBPD). DBPD is a disorder of peroxisomal fatty acid beta-oxidation. Defects in HSD17B4 are the cause of Perrault syndrome (PRS). PRS is a sex-influenced disorder characterized by sensorineural deafness in both males and females and ovarian dysgenesis in females. Some patients also have neurologic manifestations, including mild mental retardation and cerebellar and peripheral nervous system involvement. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Mitochondrial; EC 4.2.1.119; EC 1.1.1.n12; EC 4.2.1.107; Lipid Metabolism - primary bile acid biosynthesis; Oxidoreductase; Lyase; Cell development/differentiation

Cellular Component: peroxisomal membrane; membrane; mitochondrion; intracellular membrane-bound organelle; peroxisome

Molecular Function: 3(or 17)beta-hydroxysteroid dehydrogenase activity; protein homodimerization activity; 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity; isomerase activity; long-chain-enoyl-CoA hydratase activity; 3-hydroxyacyl-CoA dehydrogenase activity; receptor binding

Biological Process: osteoblast differentiation; estrogen metabolic process; cholesterol metabolic process; fatty acid beta-oxidation; metabolic process; very-long-chain fatty acid metabolic process; androgen metabolic process; Sertoli cell development
Show more

Research Articles on HSD17B4

1. Peroxisomal multifunctional protein 2 is essential for lipid homeostasis in Sertoli cells and male fertility

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HSD17B4 sirna

 Reference Product  PubMed Publications
 Scp2 sirna  >1 publications with HSD17B4 and Scp2
 Acox3 sirna  >1 publications with HSD17B4 and Acox3
 Acox1 sirna  >1 publications with HSD17B4 and Acox1

Pathways associated with HSD17B4 sirna

 Products by Pathway  Pathway Diagram
 Beta-oxidation Of Pristanoyl-CoA Pathway antibodies  Beta-oxidation Of Pristanoyl-CoA Pathway Diagram
 Beta-oxidation Of Very Long Chain Fatty Acids Pathway antibodies  Beta-oxidation Of Very Long Chain Fatty Acids Pathway Diagram
 Bile Acid And Bile Salt Metabolism Pathway antibodies  Bile Acid And Bile Salt Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Peroxisomal Lipid Metabolism Pathway antibodies  Peroxisomal Lipid Metabolism Pathway Diagram
 Peroxisome Pathway antibodies  Peroxisome Pathway Diagram
 Peroxisome Pathway antibodies  Peroxisome Pathway Diagram
 Primary Bile Acid Biosynthesis Pathway antibodies  Primary Bile Acid Biosynthesis Pathway Diagram

Diseases associated with HSD17B4 sirna

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >40 publications with HSD17B4 and Nervous System Diseases
 Peroxisomal Disorders Antibodies  >35 publications with HSD17B4 and Peroxisomal Disorders
 D-BIFUNCTIONAL PROTEIN DEFICIENCY Antibodies  >35 publications with HSD17B4 and D-BIFUNCTIONAL PROTEIN DEFICIENCY
 Brain Diseases Antibodies  >30 publications with HSD17B4 and Brain Diseases
 Liver Diseases Antibodies  >14 publications with HSD17B4 and Liver Diseases
 Zellweger Syndrome Antibodies  >12 publications with HSD17B4 and Zellweger Syndrome
 Fatty Liver Antibodies  >10 publications with HSD17B4 and Fatty Liver
 Kidney Diseases Antibodies  >9 publications with HSD17B4 and Kidney Diseases
 Breast Neoplasms Antibodies  >7 publications with HSD17B4 and Breast Neoplasms
 Gonadal dysgenesis XX type deafness Antibodies  >5 publications with HSD17B4 and Gonadal dysgenesis XX type deafness

Organs/Tissues associated with HSD17B4 sirna

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >29 publications with HSD17B4 and Brain
 Liver Antibodies  >29 publications with HSD17B4 and Liver
 Prostate Antibodies  >9 publications with HSD17B4 and Prostate
 Ovary Antibodies  >5 publications with HSD17B4 and Ovary
 Muscle Antibodies  >5 publications with HSD17B4 and Muscle
 Kidney Antibodies  >4 publications with HSD17B4 and Kidney
 Nerve Antibodies  >3 publications with HSD17B4 and Nerve
 Embryonic Tissue Antibodies  >3 publications with HSD17B4 and Embryonic Tissue
 Heart Antibodies  >2 publications with HSD17B4 and Heart
 Eye Antibodies  >2 publications with HSD17B4 and Eye
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