MAPRE1 siRNA – Microtubule-Associated Protein Rp/Eb Family Member 1 (Rat)

Name: MAPRE1 siRNA; Rat; MyBioSource
Brand: MyBioSource
SKU: MBS827772
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS827772

MBS827772 is an siRNA targeting MAPRE1. Also known as MAPRE1 siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit MAPRE1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MAPRE1, siRNA

Full Product Name

MAPRE1 siRNA (Rat)

Product Gene Name

MAPRE1 sirna

Product Synonym Names

Microtubule-associated protein RP/EB family member 1; APC-binding protein EB1; End-binding protein 1; EB1

Host

Synthetic

Species Reactivity

Rat

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

MAPRE1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

30,004 Da

3D Structure

ModBase 3D Structure for Q66HR2

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat MAPRE1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for MAPRE1 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of MAPRE1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAPRE1. It may not necessarily be applicable to this product.

NCBI GI #

78097100

NCBI GeneID

114764

NCBI Accession #

NP_612518.2

UniProt Primary Accession #

Q66HR2

UniProt Related Accession #

Q66HR2

GenBank Nucleotide #

NM_138509.3

NCBI Official Full Name

microtubule-associated protein RP/EB family member 1

NCBI Official Synonym Full Names

microtubule-associated protein, RP/EB family, member 1

NCBI Official Symbol

Mapre1

NCBI Official Synonym Symbols

Eb1

UniProt Protein Name

Microtubule-associated protein RP/EB family member 1

UniProt Synonym Protein Names

APC-binding protein EB1; End-binding protein 1; EB1

Protein Family

Microtubule-associated protein RP/EB family

UniProt Gene Name

Mapre1

UniProt Synonym Gene Names

EB1

UniProt Entry Name

MARE1_RAT

NCBI Protein Information

microtubule-associated protein RP/EB family member 1

NCBI Summary for MAPRE1

expressed specifically at growing microtubule distal tips [RGD, Feb 2006]

UniProt Comments for MAPRE1

EB1: a microtubule-associated protein of the RP/EB family. Binds to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. Localizes to microtubules, especially the growing ends, in interphase cells. Associates with the centrosomes and spindle microtubules during mitosis. Also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. May be involved in the regulation of microtubule structures and chromosome stability.

Protein type: Microtubule-binding; Cell cycle regulation; Cytoskeletal; Motility/polarity/chemotaxis

Cellular Component: microtubule cytoskeleton; Golgi apparatus; microtubule; centrosome; cell projection; cytoplasmic microtubule; cell projection membrane; cytoplasm; microtubule organizing center; cortical microtubule cytoskeleton; spindle

Molecular Function: microtubule plus-end binding

Biological Process: mitosis; cell division; negative regulation of microtubule polymerization; regulation of microtubule polymerization

Research Articles on MAPRE1

1. provides a functional link between tubulin- and actin-based cytoskeletons to confer spermatocyte transport at the blood-testis barrier

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MAPRE1 sirna

Reference Product	PubMed Publications
Aurkb sirna	>3 publications with MAPRE1 and Aurkb

Pathways associated with MAPRE1 sirna

Products by Pathway	Pathway Diagram
Anchoring Of The Basal Body To The Plasma Membrane Pathway antibodies	Anchoring Of The Basal Body To The Plasma Membrane Pathway Diagram
Assembly Of The Primary Cilium Pathway antibodies	Assembly Of The Primary Cilium Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Centrosome Maturation Pathway antibodies	Centrosome Maturation Pathway Diagram
G2/M Transition Pathway antibodies	G2/M Transition Pathway Diagram
Loss Of Nlp From Mitotic Centrosomes Pathway antibodies	Loss Of Nlp From Mitotic Centrosomes Pathway Diagram
Loss Of Proteins Required For Interphase Microtubule Organizationfrom The Centrosome Pathway antibodies	Loss Of Proteins Required For Interphase Microtubule Organizationfrom The Centrosome Pathway Diagram
M Phase Pathway antibodies	M Phase Pathway Diagram
Mitotic Anaphase Pathway antibodies	Mitotic Anaphase Pathway Diagram

Diseases associated with MAPRE1 sirna

Disease Name	Pubmed Publications
Carcinoma Antibodies	>8 publications with MAPRE1 and Carcinoma
Breast Neoplasms Antibodies	>3 publications with MAPRE1 and Breast Neoplasms
Nervous System Diseases Antibodies	>3 publications with MAPRE1 and Nervous System Diseases
Adenocarcinoma Antibodies	>3 publications with MAPRE1 and Adenocarcinoma
Carcinoma, Hepatocellular Antibodies	>2 publications with MAPRE1 and Carcinoma, Hepatocellular
Inflammation Antibodies	>2 publications with MAPRE1 and Inflammation
Lung Diseases Antibodies	>2 publications with MAPRE1 and Lung Diseases
Brain Diseases Antibodies	>2 publications with MAPRE1 and Brain Diseases
Lung Neoplasms Antibodies	>2 publications with MAPRE1 and Lung Neoplasms
Hypertrophy Antibodies	>1 publications with MAPRE1 and Hypertrophy

Organs/Tissues associated with MAPRE1 sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>6 publications with MAPRE1 and Brain
Lung Antibodies	>4 publications with MAPRE1 and Lung
Heart Antibodies	>4 publications with MAPRE1 and Heart
Muscle Antibodies	>3 publications with MAPRE1 and Muscle
Kidney Antibodies	>3 publications with MAPRE1 and Kidney
Liver Antibodies	>2 publications with MAPRE1 and Liver
Inner Ear Antibodies	>1 publications with MAPRE1 and Inner Ear
Eye Antibodies	>1 publications with MAPRE1 and Eye
Colon Antibodies	>1 publications with MAPRE1 and Colon
Dorsal Root Ganglion Antibodies	>1 publications with MAPRE1 and Dorsal Root Ganglion