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OCLN siRNA – Occludin (Rat)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828404

MBS828404 is an siRNA targeting OCLN. Also known as OCLN siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit OCLN expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
OCLN, siRNA
Full Product Name
OCLN siRNA (Rat)
Product Gene Name
OCLN sirna
Product Synonym Names
Occludin
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
OCLN siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
59,186 Da
3D Structure
ModBase 3D Structure for Q6P6T5
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat OCLN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for OCLN sirna
RNA Interference (RNAi)

Other Notes

Small volumes of OCLN sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OCLN. It may not necessarily be applicable to this product.

NCBI GI #
40254754
NCBI GeneID
83497
NCBI Accession #
NP_112619.2
UniProt Primary Accession #
Q6P6T5
UniProt Secondary Accession #
Q9Z303
UniProt Related Accession #
Q6P6T5
GenBank Nucleotide #
NM_031329.2
NCBI Official Full Name
occludin
NCBI Official Synonym Full Names
occludin
NCBI Official Symbol
Ocln
UniProt Protein Name
Occludin
Protein Family
Occludin
UniProt Gene Name
Ocln
UniProt Entry Name
OCLN_RAT
NCBI Protein Information
occludin

UniProt Comments for OCLN

occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Cellular Component: tight junction; apicolateral plasma membrane; endocytic vesicle; apical plasma membrane; integral to membrane; plasma membrane; intercellular junction; cytoplasmic vesicle; cytosol; cell junction

Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; S-adenosylmethionine-dependent methyltransferase activity; structural molecule activity

Biological Process: methylation; intercellular junction assembly and maintenance; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process
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Research Articles on OCLN

1. The data indicated that early exercise significantly inhibited the ischemia-induced reduction of occludin, and an increase in MMP-9 promoted TIMP-1 expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with OCLN sirna

 Reference Product  PubMed Publications
 Tjp1 sirna  >543 publications with OCLN and Tjp1
 Cldn1 sirna  >279 publications with OCLN and Cldn1
 Cldn5 sirna  >135 publications with OCLN and Cldn5
 Cldn2 sirna  >68 publications with OCLN and Cldn2
 Cldn4 sirna  >66 publications with OCLN and Cldn4
 Cldn3 sirna  >49 publications with OCLN and Cldn3
 Tjp2 sirna  >48 publications with OCLN and Tjp2
 Cldn7 sirna  >17 publications with OCLN and Cldn7
 Tjp3 sirna  >14 publications with OCLN and Tjp3

Pathways associated with OCLN sirna

 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram
 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram

Diseases associated with OCLN sirna

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >148 publications with OCLN and Inflammation
 Disease Models, Animal Antibodies  >122 publications with OCLN and Disease Models, Animal
 Necrosis Antibodies  >86 publications with OCLN and Necrosis
 Edema Antibodies  >60 publications with OCLN and Edema
 Liver Diseases Antibodies  >45 publications with OCLN and Liver Diseases
 Retinal Diseases Antibodies  >33 publications with OCLN and Retinal Diseases
 Colitis Antibodies  >30 publications with OCLN and Colitis
 Lung Diseases Antibodies  >23 publications with OCLN and Lung Diseases
 Hemorrhage Antibodies  >15 publications with OCLN and Hemorrhage
 Kidney Diseases Antibodies  >15 publications with OCLN and Kidney Diseases

Organs/Tissues associated with OCLN sirna

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >275 publications with OCLN and Brain
 Liver Antibodies  >125 publications with OCLN and Liver
 Kidney Antibodies  >73 publications with OCLN and Kidney
 Colon Antibodies  >72 publications with OCLN and Colon
 Embryonic Tissue Antibodies  >53 publications with OCLN and Embryonic Tissue
 Lung Antibodies  >48 publications with OCLN and Lung
 Placenta Antibodies  >13 publications with OCLN and Placenta
 Inner Ear Antibodies  >5 publications with OCLN and Inner Ear
 Prostate Antibodies  >2 publications with OCLN and Prostate
 Uncharacterized Tissue Antibodies  >1 publications with OCLN and Uncharacterized Tissue
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