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POLB siRNA – DNA Polymerase Beta (Rat)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8206717

MBS8206717 is an siRNA targeting POLB. Also known as POLB siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit POLB expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POLB, siRNA
Full Product Name
POLB siRNA (Rat)
Product Gene Name
POLB sirna
Product Synonym Names
DNA polymerase beta
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
POLB siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
38,327 Da
3D Structure
ModBase 3D Structure for P06766
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat POLB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for POLB sirna
RNA Interference (RNAi)

Other Notes

Small volumes of POLB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POLB. It may not necessarily be applicable to this product.

NCBI GI #
148747154
NCBI GeneID
29240
NCBI Accession #
NP_058837.2
UniProt Primary Accession #
P06766
UniProt Secondary Accession #
Q4G081
UniProt Related Accession #
P06766
GenBank Nucleotide #
NM_017141.2
NCBI Official Full Name
DNA polymerase beta
NCBI Official Synonym Full Names
polymerase (DNA directed), beta
NCBI Official Symbol
Polb
UniProt Protein Name
DNA polymerase beta
Protein Family
DNA polymerase
UniProt Gene Name
Polb
UniProt Entry Name
DPOLB_RAT
NCBI Protein Information
DNA polymerase beta

NCBI Summary for POLB

plays a role in DNA repair and bypass of pyrimidine dimers produced by UV-irradiation; also involved in base-excision repair [RGD, Feb 2006]

UniProt Comments for POLB

POLB: Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Monomer. Interacts with APEX1, HUWE1/ARF-BP1, STUB1/CHIP and USP47. Belongs to the DNA polymerase type-X family.

Protein type: Lyase; Transferase; DNA replication; DNA repair, damage; EC 2.7.7.7

Cellular Component: microtubule; spindle microtubule; cytoplasm; intracellular; nucleus

Molecular Function: protein binding; enzyme binding; DNA binding; microtubule binding; metal ion binding; DNA-directed DNA polymerase activity; damaged DNA binding; lyase activity

Biological Process: base-excision repair, gap-filling; neuron apoptosis; response to hyperoxia; response to ethanol; DNA damage response, signal transduction resulting in induction of apoptosis; base-excision repair; pyrimidine dimer repair; response to gamma radiation; nucleotide-excision repair, DNA gap filling; DNA replication; response to DNA damage stimulus; aging
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Research Articles on POLB

1. The I260Q variant of DNA polymerase beta is an efficient mutator polymerase with fairly indiscriminate misincorporation activities opposite all template bases.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with POLB sirna

 Reference Product  PubMed Publications
 Xrcc1 sirna  >13 publications with POLB and Xrcc1
 Pcna sirna  >10 publications with POLB and Pcna
 Apex1 sirna  >9 publications with POLB and Apex1
 Lig3 sirna  >9 publications with POLB and Lig3
 Pold1 sirna  >2 publications with POLB and Pold1
 Lig4 sirna  >1 publications with POLB and Lig4

Pathways associated with POLB sirna

 Products by Pathway  Pathway Diagram
 APEX1-Independent Resolution Of AP Sites Via The Single Nucleotide Replacement Pathway antibodies  APEX1-Independent Resolution Of AP Sites Via The Single Nucleotide Replacement Pathway Diagram
 Abasic Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway antibodies  Abasic Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway Diagram
 BER Complex Pathway antibodies  BER Complex Pathway Diagram
 BER Complex Pathway antibodies  BER Complex Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with POLB sirna

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >11 publications with POLB and Carcinoma
 Chromosome Aberrations Antibodies  >5 publications with POLB and Chromosome Aberrations
 Lung Neoplasms Antibodies  >5 publications with POLB and Lung Neoplasms
 Urinary Bladder Neoplasms Antibodies  >4 publications with POLB and Urinary Bladder Neoplasms
 Cell Transformation, Neoplastic Antibodies  >3 publications with POLB and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >3 publications with POLB and Breast Neoplasms
 Pancreatic Neoplasms Antibodies  >2 publications with POLB and Pancreatic Neoplasms
 Hyperplasia Antibodies  >2 publications with POLB and Hyperplasia
 Uterine Cervical Neoplasms Antibodies  >2 publications with POLB and Uterine Cervical Neoplasms
 Anemia Antibodies  >2 publications with POLB and Anemia

Organs/Tissues associated with POLB sirna

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >2 publications with POLB and Kidney
 Brain Antibodies  >2 publications with POLB and Brain
 Eye Antibodies  >1 publications with POLB and Eye
 Heart Antibodies  >1 publications with POLB and Heart
 Embryonic Tissue Antibodies  >1 publications with POLB and Embryonic Tissue
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