RIPK3 siRNA – Receptor-Interacting Serine/Threonine-Protein Kinase 3 (Rat)

Name: RIPK3 siRNA; Rat; MyBioSource
Brand: MyBioSource
SKU: MBS8218005
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8218005

MBS8218005 is an siRNA targeting RIPK3. Also known as RIPK3 siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit RIPK3 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RIPK3, siRNA

Full Product Name

RIPK3 siRNA (Rat)

Product Gene Name

RIPK3 sirna

Product Synonym Names

RIP3; Receptor-interacting serine/threonine-protein kinase 3; Homocysteine respondent protein HCYP2; RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3

Host

Synthetic

Species Reactivity

Rat

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

RIPK3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

52,218 Da

3D Structure

ModBase 3D Structure for Q9Z2P5

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat RIPK3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for RIPK3 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of RIPK3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RIPK3. It may not necessarily be applicable to this product.

NCBI GI #

21326477

NCBI GeneID

246240

NCBI Accession #

NP_647558.1

UniProt Primary Accession #

Q9Z2P5

UniProt Secondary Accession #

B0BMV6

UniProt Related Accession #

Q9Z2P5

GenBank Nucleotide #

NM_139342.1

NCBI Official Full Name

receptor-interacting serine/threonine-protein kinase 3

NCBI Official Synonym Full Names

receptor-interacting serine-threonine kinase 3

NCBI Official Symbol

Ripk3

NCBI Official Synonym Symbols

Rip3; Hcyp2

UniProt Protein Name

Receptor-interacting serine/threonine-protein kinase 3

UniProt Synonym Protein Names

Homocysteine respondent protein HCYP2; RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3

Protein Family

Receptor-interacting serine/threonine-protein kinase

UniProt Gene Name

Ripk3

UniProt Synonym Gene Names

Rip3; RIP-3

UniProt Entry Name

RIPK3_RAT

NCBI Protein Information

receptor-interacting serine/threonine-protein kinase 3

NCBI Summary for RIPK3

a putative homocysteine respondent protein [RGD, Feb 2006]

UniProt Comments for RIPK3

RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, TKL; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); TKL group; RIPK family

Cellular Component: mitochondrion; plasma membrane; intracellular; cytosol

Molecular Function: NF-kappaB-inducing kinase activity; protein serine/threonine kinase activity; identical protein binding; protein complex binding; ATP binding; protein kinase activity

Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; regulation of activated T cell proliferation; thymus development; protein heterooligomerization; protein amino acid autophosphorylation; T cell differentiation in the thymus; positive regulation of transferase activity; lymph node development; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; regulation of T cell mediated cytotoxicity; regulation of adaptive immune response; regulation of interferon-gamma production; activation of protein kinase activity; T cell homeostasis; positive regulation of ligase activity; protein homooligomerization; positive regulation of oxidoreductase activity

Research Articles on RIPK3

1. These results indicated that necroptosis mediated by RIP1 and RIP3 participates in the loss of renal cells of subtotal nephrectomised rats.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with RIPK3 sirna

Reference Product	PubMed Publications
Ripk1 sirna	>115 publications with RIPK3 and Ripk1
Mlkl sirna	>71 publications with RIPK3 and Mlkl
Rela sirna	>3 publications with RIPK3 and Rela

Pathways associated with RIPK3 sirna

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Cytosolic DNA-sensing Pathway antibodies	Cytosolic DNA-sensing Pathway Diagram
Cytosolic DNA-sensing Pathway antibodies	Cytosolic DNA-sensing Pathway Diagram
Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies	Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
IKK Complex Recruitment Mediated By RIP1 Pathway antibodies	IKK Complex Recruitment Mediated By RIP1 Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
MyD88-independent TLR3/TLR4 Cascade Pathway antibodies	MyD88-independent TLR3/TLR4 Cascade Pathway Diagram
Programmed Cell Death Pathway antibodies	Programmed Cell Death Pathway Diagram
RIP-mediated NFkB Activation Via ZBP1 Pathway antibodies	RIP-mediated NFkB Activation Via ZBP1 Pathway Diagram

Diseases associated with RIPK3 sirna

Disease Name	Pubmed Publications
Necrosis Antibodies	>227 publications with RIPK3 and Necrosis
Inflammation Antibodies	>89 publications with RIPK3 and Inflammation
Cardiovascular Diseases Antibodies	>19 publications with RIPK3 and Cardiovascular Diseases
Vascular Diseases Antibodies	>19 publications with RIPK3 and Vascular Diseases
Liver Diseases Antibodies	>17 publications with RIPK3 and Liver Diseases
Drug-Induced Liver Injury Antibodies	>8 publications with RIPK3 and Drug-Induced Liver Injury
Heart Diseases Antibodies	>5 publications with RIPK3 and Heart Diseases
Drug-Related Side Effects and Adverse Reactions Antibodies	>5 publications with RIPK3 and Drug-Related Side Effects and Adverse Reactions
Lung Diseases Antibodies	>5 publications with RIPK3 and Lung Diseases
Acute Kidney Injury Antibodies	>4 publications with RIPK3 and Acute Kidney Injury

Organs/Tissues associated with RIPK3 sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>14 publications with RIPK3 and Brain
Embryonic Tissue Antibodies	>10 publications with RIPK3 and Embryonic Tissue
Heart Antibodies	>9 publications with RIPK3 and Heart
Muscle Antibodies	>5 publications with RIPK3 and Muscle
Small Intestine Antibodies	>4 publications with RIPK3 and Small Intestine
Artery Antibodies	>3 publications with RIPK3 and Artery
Spleen Antibodies	>3 publications with RIPK3 and Spleen
Placenta Antibodies	>1 publications with RIPK3 and Placenta
Thymus Antibodies	>1 publications with RIPK3 and Thymus
Prostate Antibodies	>1 publications with RIPK3 and Prostate