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SMPD3 siRNA – Sphingomyelin Phosphodiesterase 3 (Rat)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8234013

MBS8234013 is an siRNA targeting SMPD3. Also known as SMPD3 siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit SMPD3 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SMPD3, siRNA
Full Product Name
SMPD3 siRNA (Rat)
Product Gene Name
SMPD3 sirna
Product Synonym Names
CCA1; Sphingomyelin phosphodiesterase 3; Confluent 3Y1 cell-associated protein 1; Neutral sphingomyelinase 2; nSMase-2; nSMase2; Neutral sphingomyelinase II
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
SMPD3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
71,272 Da
3D Structure
ModBase 3D Structure for O35049
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SMPD3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for SMPD3 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of SMPD3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SMPD3. It may not necessarily be applicable to this product.

NCBI GI #
16758394
NCBI GeneID
94338
NCBI Accession #
NP_446057.1
UniProt Primary Accession #
O35049
UniProt Related Accession #
O35049
GenBank Nucleotide #
NM_053605.1
NCBI Official Full Name
sphingomyelin phosphodiesterase 3
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase 3, neutral membrane
NCBI Official Symbol
Smpd3
NCBI Official Synonym Symbols
cca1
UniProt Protein Name
Sphingomyelin phosphodiesterase 3
UniProt Synonym Protein Names
Confluent 3Y1 cell-associated protein 1; Neutral sphingomyelinase 2; nSMase-2; nSMase2; Neutral sphingomyelinase II
Protein Family
Sphingomyelin phosphodiesterase
UniProt Gene Name
Smpd3
UniProt Synonym Gene Names
Cca1; nSMase-2; nSMase2
UniProt Entry Name
NSMA2_RAT
NCBI Protein Information
sphingomyelin phosphodiesterase 3

NCBI Summary for SMPD3

mRNA accumulation occurs in a growth arrested confluent monolayer but not in subconfluent cells; may play a role in regulation of cell growth [RGD, Feb 2006]

UniProt Comments for SMPD3

SMPD3: Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization. Belongs to the neutral sphingomyelinase family.

Protein type: Lipid Metabolism - sphingolipid; EC 3.1.4.12; Membrane protein, integral; Phospholipase

Cellular Component: Golgi membrane; Golgi apparatus; cell; plasma membrane; Golgi cis cisterna

Molecular Function: protein binding; sphingomyelin phosphodiesterase activity; metal ion binding

Biological Process: sphingolipid metabolic process; sphingomyelin metabolic process; multicellular organismal development; hemopoietic progenitor cell differentiation; peptide hormone secretion
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Research Articles on SMPD3

1. The results of this study reveal that cerebral ischemia induced the activation of the nSMase2/ceramide pathway in astrocytes, but not neurons in the rat hippocampus

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SMPD3 sirna

 Reference Product  PubMed Publications
 Smpd1 sirna  >85 publications with SMPD3 and Smpd1
 Asah2 sirna  >1 publications with SMPD3 and Asah2
 Smpd2 sirna  >1 publications with SMPD3 and Smpd2

Pathways associated with SMPD3 sirna

 Products by Pathway  Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram

Diseases associated with SMPD3 sirna

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >16 publications with SMPD3 and Necrosis
 Inflammation Antibodies  >10 publications with SMPD3 and Inflammation
 Disease Models, Animal Antibodies  >8 publications with SMPD3 and Disease Models, Animal
 Fatty Liver Antibodies  >3 publications with SMPD3 and Fatty Liver
 Cardiovascular Diseases Antibodies  >3 publications with SMPD3 and Cardiovascular Diseases
 Liver Diseases Antibodies  >2 publications with SMPD3 and Liver Diseases
 Hyperplasia Antibodies  >2 publications with SMPD3 and Hyperplasia
 Fetal Death Antibodies  >2 publications with SMPD3 and Fetal Death
 Learning Disorders Antibodies  >1 publications with SMPD3 and Learning Disorders
 Carcinoma, Hepatocellular Antibodies  >1 publications with SMPD3 and Carcinoma, Hepatocellular

Organs/Tissues associated with SMPD3 sirna

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >13 publications with SMPD3 and Brain
 Connective Tissue Antibodies  >3 publications with SMPD3 and Connective Tissue
 Prostate Antibodies  >2 publications with SMPD3 and Prostate
 Adipose Tissue Antibodies  >2 publications with SMPD3 and Adipose Tissue
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