TBCE siRNA – Tubulin-Specific Chaperone E (Rat)

Name: TBCE siRNA; Tubulin-Specific Chaperone E; Rat; MyBioSource
Brand: MyBioSource
SKU: MBS8224540
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8224540

MBS8224540 is an siRNA targeting TBCE. Also known as TBCE siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit TBCE expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TBCE, siRNA

Full Product Name

TBCE siRNA (Rat)

Product Gene Name

TBCE sirna

Product Synonym Names

Tubulin-specific chaperone E; Tubulin-folding cofactor E

Host

Synthetic

Species Reactivity

Rat

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

TBCE siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

59,043 Da

3D Structure

ModBase 3D Structure for Q5FVQ9

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat TBCE gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for TBCE sirna

RNA Interference (RNAi)

Other Notes

Small volumes of TBCE sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TBCE. It may not necessarily be applicable to this product.

NCBI GI #

58865894

NCBI GeneID

361255

NCBI Accession #

NP_001012161.1

UniProt Primary Accession #

Q5FVQ9

UniProt Related Accession #

Q5FVQ9

GenBank Nucleotide #

NM_001012161.1

NCBI Official Full Name

tubulin-specific chaperone E

NCBI Official Synonym Full Names

tubulin folding cofactor E

NCBI Official Symbol

Tbce

UniProt Protein Name

Tubulin-specific chaperone E

UniProt Synonym Protein Names

Tubulin-folding cofactor E

Protein Family

Tubulin-specific chaperone

UniProt Gene Name

Tbce

UniProt Entry Name

TBCE_RAT

NCBI Protein Information

tubulin-specific chaperone E

UniProt Comments for TBCE

TBCE: Tubulin-folding protein; involved in the second step of the tubulin folding pathway. Seems to be implicated in the maintenance of the neuronal microtubule network. Involved in regulation of tubulin heterodimer dissociation. Defects in TBCE are a cause of hypoparathyroidism- retardation-dysmorphism syndrome (HRD); also known as hypoparathyroidism with short stature, mental retardation, and seizures or Sanjad-Sakati syndrome. HRD is an autosomal recessive disorder reported almost exclusively in Middle Eastern populations. Defects in TBCE are the cause of Kenny-Caffey syndrome type 1 (KCS1). KCS1 is similar to HRD with the additional features of osteosclerosis and recurrent bacterial infections. Belongs to the TBCE family.

Protein type: Chaperone

Cellular Component: cytoskeleton; cytoplasm

Biological Process: developmental growth; axonogenesis; adult locomotory behavior; muscle atrophy; microtubule cytoskeleton organization and biogenesis; post-chaperonin tubulin folding pathway; peripheral nervous system neuron axonogenesis; post-embryonic development

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TBCE sirna

Reference Product	PubMed Publications
Tbcc sirna	>6 publications with TBCE and Tbcc
Tbca sirna	>6 publications with TBCE and Tbca
Tbcd sirna	>6 publications with TBCE and Tbcd
Tbcb sirna	>5 publications with TBCE and Tbcb

Pathways associated with TBCE sirna

Products by Pathway	Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Post-chaperonin Tubulin Folding Pathway antibodies	Post-chaperonin Tubulin Folding Pathway Diagram
Protein Folding Pathway antibodies	Protein Folding Pathway Diagram

Diseases associated with TBCE sirna

Disease Name	Pubmed Publications
Neurobehavioral Manifestations Antibodies	>13 publications with TBCE and Neurobehavioral Manifestations
Kenny Caffey syndrome Antibodies	>7 publications with TBCE and Kenny Caffey syndrome
Hypoparathyroidism-retardation-dysmorphism syndrome Antibodies	>3 publications with TBCE and Hypoparathyroidism-retardation-dysmorphism syndrome
Facies Antibodies	>3 publications with TBCE and Facies
Atrophy Antibodies	>3 publications with TBCE and Atrophy
Nerve Degeneration Antibodies	>3 publications with TBCE and Nerve Degeneration
Fetal Growth Retardation Antibodies	>3 publications with TBCE and Fetal Growth Retardation
Kenny-Caffey syndrome, Type 1 Antibodies	>2 publications with TBCE and Kenny-Caffey syndrome, Type 1
Bacterial Infections Antibodies	>2 publications with TBCE and Bacterial Infections
Craniofacial Abnormalities Antibodies	>2 publications with TBCE and Craniofacial Abnormalities

Organs/Tissues associated with TBCE sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>5 publications with TBCE and Brain
Eye Antibodies	>3 publications with TBCE and Eye
Inner Ear Antibodies	>2 publications with TBCE and Inner Ear
Ovary Antibodies	>1 publications with TBCE and Ovary
Testis Antibodies	>1 publications with TBCE and Testis
Pituitary Gland Antibodies	>1 publications with TBCE and Pituitary Gland
Embryonic Tissue Antibodies	>1 publications with TBCE and Embryonic Tissue