USP7 siRNA – Ubiquitin Carboxyl-Terminal Hydrolase 7 (Rat)

Name: USP7 siRNA; Ubiquitin Carboxyl-Terminal Hydrolase 7; Rat; MyBioSource
Brand: MyBioSource
SKU: MBS8202439
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8202439

MBS8202439 is an siRNA targeting USP7. Also known as USP7 siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit USP7 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

USP7, siRNA

Full Product Name

USP7 siRNA (Rat)

Product Gene Name

USP7 sirna

Product Synonym Names

HAUSP; Ubiquitin carboxyl-terminal hydrolase 7; Deubiquitinating enzyme 7; Herpesvirus-associated ubiquitin-specific protease; rHAUSP; Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7

Host

Synthetic

Species Reactivity

Rat

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

USP7 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

128,431 Da

3D Structure

ModBase 3D Structure for Q4VSI4

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat USP7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for USP7 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of USP7 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for USP7. It may not necessarily be applicable to this product.

NCBI GI #

67078438

NCBI GeneID

360471

NCBI Accession #

NP_001019961.1

UniProt Primary Accession #

Q4VSI4

UniProt Related Accession #

Q4VSI4

GenBank Nucleotide #

NM_001024790.1

NCBI Official Full Name

ubiquitin carboxyl-terminal hydrolase 7

NCBI Official Synonym Full Names

ubiquitin specific peptidase 7 (herpes virus-associated)

NCBI Official Symbol

Usp7

NCBI Official Synonym Symbols

Hausp

UniProt Protein Name

Ubiquitin carboxyl-terminal hydrolase 7

UniProt Synonym Protein Names

Deubiquitinating enzyme 7; Herpesvirus-associated ubiquitin-specific protease; rHAUSP; Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7

UniProt Gene Name

Usp7

UniProt Synonym Gene Names

Hausp; rHAUSP

UniProt Entry Name

UBP7_RAT

NCBI Protein Information

ubiquitin carboxyl-terminal hydrolase 7

UniProt Comments for USP7

USP7: an enzyme that removes ubiquitin from its protein substrates. Binds to the herpes virus protein VMW110 that may therefore modulate its substrate specificity or activity to stabilize viral proteins. Regulates MDM2 and has a dynamic role in the p53-MDM2 pathway. Is reported to deubiquitinate and stabilize p53.

Protein type: Ubiquitin-specific protease; Ubiquitin conjugating system; Protease; EC 3.4.19.12

Cellular Component: nuclear body; PML body; cytoplasm; cytosol; nucleus

Molecular Function: protein C-terminus binding; protein homodimerization activity; p53 binding; cysteine-type endopeptidase activity; ubiquitin protein ligase binding; ubiquitin-specific protease activity; transcription factor binding

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; maintenance of DNA methylation; positive regulation of apoptosis; multicellular organismal development; regulation of protein stability; proteolysis; histone deubiquitination; regulation of transcription, DNA-dependent; regulation of transcription factor activity; inhibition of NF-kappaB transcription factor; transcription-coupled nucleotide-excision repair

Research Articles on USP7

1. A rat HAUSP from testis was identified and cloned.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with USP7 sirna

Reference Product	PubMed Publications
Mdm2 sirna	>52 publications with USP7 and Mdm2
Tp53 sirna	>11 publications with USP7 and Tp53
Dnmt1 sirna	>7 publications with USP7 and Dnmt1
Mdm4 sirna	>6 publications with USP7 and Mdm4
Traf6 sirna	>3 publications with USP7 and Traf6
Usp11 sirna	>1 publications with USP7 and Usp11
Ikbkg sirna	>1 publications with USP7 and Ikbkg

Pathways associated with USP7 sirna

Products by Pathway	Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram
FoxO Signaling Pathway antibodies	FoxO Signaling Pathway Diagram
Herpes Simplex Infection Pathway antibodies	Herpes Simplex Infection Pathway Diagram
Herpes Simplex Infection Pathway antibodies	Herpes Simplex Infection Pathway Diagram
Viral Carcinogenesis Pathway antibodies	Viral Carcinogenesis Pathway Diagram
Viral Carcinogenesis Pathway antibodies	Viral Carcinogenesis Pathway Diagram

Diseases associated with USP7 sirna

Disease Name	Pubmed Publications
Death Antibodies	>18 publications with USP7 and Death
Necrosis Antibodies	>12 publications with USP7 and Necrosis
Nervous System Diseases Antibodies	>9 publications with USP7 and Nervous System Diseases
Cell Transformation, Neoplastic Antibodies	>8 publications with USP7 and Cell Transformation, Neoplastic
Lung Neoplasms Antibodies	>8 publications with USP7 and Lung Neoplasms
Inflammation Antibodies	>6 publications with USP7 and Inflammation
Neoplasms, Experimental Antibodies	>5 publications with USP7 and Neoplasms, Experimental
Cardiovascular Diseases Antibodies	>5 publications with USP7 and Cardiovascular Diseases
Lung Diseases Antibodies	>4 publications with USP7 and Lung Diseases
Prostatic Diseases Antibodies	>3 publications with USP7 and Prostatic Diseases

Organs/Tissues associated with USP7 sirna

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>14 publications with USP7 and Lung
Prostate Antibodies	>8 publications with USP7 and Prostate
Liver Antibodies	>6 publications with USP7 and Liver
Kidney Antibodies	>5 publications with USP7 and Kidney
Brain Antibodies	>5 publications with USP7 and Brain
Testis Antibodies	>4 publications with USP7 and Testis
Heart Antibodies	>2 publications with USP7 and Heart
Muscle Antibodies	>2 publications with USP7 and Muscle
Embryonic Tissue Antibodies	>2 publications with USP7 and Embryonic Tissue
Connective Tissue Antibodies	>1 publications with USP7 and Connective Tissue