ACOT8 Recombinant Protein

Name: ACOT8 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS205417
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205417

Acyl-coenzyme A thioesterase 8, also known as ACOT8, are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and… More details

$230 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ACOT8, Recombinant Protein

Full Product Name

ACOT8, 1-319aa, Human, His tag, E Coli

Product Gene Name

ACOT8 recombinant protein

Product Synonym Names

Acyl-coenzyme A thioesterase 8; hACTE-III; HNAACTE; the; PTE-1; PTE-2; PTE1; PTE2

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSSPQAP EDGQGCGDRG DPPGDLRSVL VTTVLNLEPL DEDLFRGRHY WVPAKRLFGG QIVGQALVAA AKSVSEDVHV HSLHCYFVRA GDPKLPVLYQ VERTRTGSSF SVRSVKAVQH GKPIFICQAS FQQAQPSPMQ HQFSMPTVPP PEELLDCETL IDQYLRDPNL QKRYPLALNR IAAQEVPIEI KPVNPSPLSQ LQRMEPKQMF WVRARGYIGE GDMKMHCCVA AYISDYAFLG TALLPHQWQH KVHFMVSLDH SMWFHAPFRA DHWMLYECES PWAGGSRGLV HGRLWRQDGV LAVTCAQEGV IRVKPQVSES KL

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

38.3 kDa (342aa), confirmed by MALDI-TOF

OMIM

AF014404 mRNA

3D Structure

ModBase 3D Structure for O14734

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for ACOT8 recombinant protein

Metabolism

Applications Tested/Suitable for ACOT8 recombinant protein

SDS-PAGE

Product References and Citations for ACOT8 recombinant protein

Ishizuka M., et al. (2004) Exp. Cell Res. 297:127-141; Hunt M.C., et al. (2002) Prog. Lipid Res. 41:99-130

Other Notes

Small volumes of ACOT8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACOT8 purchase

MBS205417 is a recombinant protein targeting ACOT8. Also known as ACOT8, 1-319aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ACOT8. It may not necessarily be applicable to this product.

NCBI GI #

34577075

NCBI GeneID

10005

NCBI Accession #

NP_005460

UniProt Primary Accession #

O14734

UniProt Secondary Accession #

O15261; Q17RX4

UniProt Related Accession #

O14734

GenBank Nucleotide #

NM_005469.3

NCBI Official Full Name

acyl-coenzyme A thioesterase 8

NCBI Official Synonym Full Names

acyl-CoA thioesterase 8

NCBI Official Symbol

ACOT8

NCBI Official Synonym Symbols

hTE; NAP1; PTE1; PTE2; PTE-1; PTE-2; HNAACTE; hACTE-III

UniProt Protein Name

Acyl-coenzyme A thioesterase 8

UniProt Synonym Protein Names

Choloyl-coenzyme A thioesterase; HIV-Nef-associated acyl-CoA thioesterase; PTE-2; Peroxisomal acyl-coenzyme A thioester hydrolase 1; PTE-1; Peroxisomal long-chain acyl-CoA thioesterase 1; Thioesterase II; hACTE-III; hACTEIII; hTE

Protein Family

Acyl-coenzyme A thioesterase

UniProt Gene Name

ACOT8

UniProt Synonym Gene Names

ACTEIII; PTE1; PTE2; Acyl-CoA thioesterase 8; PTE-1; hACTE-III; hACTEIII; hTE

NCBI Protein Information

acyl-coenzyme A thioesterase 8

NCBI Summary for ACOT8

The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010]

UniProt Comments for ACOT8

Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:9299485, PubMed:9153233, PubMed:15194431). Competes with bile acid CoA:amino acid N-acyltransferase (BAAT) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (C2 to C20) (PubMed:9299485, PubMed:9153233). Inactive towards substrates with more than C20 aliphatic chains (PubMed:9153233). Involved in the metabolic regulation of peroxisome proliferation (PubMed:15194431).

Research Articles on ACOT8

1. In summary, lipolytic enzyme ACOT8 is frequently upregulated in HCC clinical specimens. More importantly, ACOT8 silencing leads to inhibition of cell growth in HCC in vitro.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ACOT8 recombinant protein

Reference Product	PubMed Publications
BAAT recombinant protein	>1 publications with ACOT8 and BAAT

Pathways associated with ACOT8 recombinant protein

Products by Pathway	Pathway Diagram
Beta-oxidation Of Pristanoyl-CoA Pathway antibodies	Beta-oxidation Of Pristanoyl-CoA Pathway Diagram
Beta-oxidation Of Very Long Chain Fatty Acids Pathway antibodies	Beta-oxidation Of Very Long Chain Fatty Acids Pathway Diagram
Bile Acid And Bile Salt Metabolism Pathway antibodies	Bile Acid And Bile Salt Metabolism Pathway Diagram
Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway antibodies	Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway Diagram
Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway antibodies	Bile Acid Biosynthesis, Cholesterol => Cholate/chenodeoxycholate Pathway Diagram
Fatty Acyl-CoA Biosynthesis Pathway antibodies	Fatty Acyl-CoA Biosynthesis Pathway Diagram
Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies	Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram

Diseases associated with ACOT8 recombinant protein

Disease Name	Pubmed Publications
Fatty Liver Antibodies	>3 publications with ACOT8 and Fatty Liver
Liver Diseases Antibodies	>2 publications with ACOT8 and Liver Diseases
Congenital, Hereditary, and Neonatal Diseases and Abnormalities Antibodies	>1 publications with ACOT8 and Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Carcinoma, Hepatocellular Antibodies	>1 publications with ACOT8 and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>1 publications with ACOT8 and Liver Neoplasms

Organs/Tissues associated with ACOT8 recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>3 publications with ACOT8 and Blood
Liver Antibodies	>3 publications with ACOT8 and Liver
Thyroid Antibodies	>1 publications with ACOT8 and Thyroid
Lymph Antibodies	>1 publications with ACOT8 and Lymph
Kidney Antibodies	>1 publications with ACOT8 and Kidney
Lung Antibodies	>1 publications with ACOT8 and Lung
Lymph Node Antibodies	>1 publications with ACOT8 and Lymph Node