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Beta-Nerve Growth Factor (NGF) Rat Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9021601

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systs. Extracellular domain ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to… More details

$350 / 0.02 mg (Mammalian-Cell) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Beta-nerve growth factor (Ngf), Recombinant Protein
Full Product Name
Recombinant Rat Beta-nerve growth factor (Ngf)
Product Gene Name
Ngf recombinant protein
Product Synonym Names
Beta-NGF
Sequence
EPYTDSNVPE GDSVPEAHWT KLQHSLDTAL RRARSAPAEP IAARVTGQTR NITVDPKLFK KRRLRSPRVL FSTQPPPTSS DTLDLDFQAH GTISFNRTHR SKRSSTHPVF HMGEFSVCDS VSVWVGDKTT ATDIKGKEVT VLGEVNINNS VFKQYFFETK CRAPNPVESG CRGIDSKHWN SYCTTTHTFV KALTTDDKQA AWRFIRIDTA CVCVLSRKAA RRG
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Liquid or Lyophilized powder
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.
Purity/Purification
Greater than 90% as determined by SDS-PAGE. (lot specific)
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
Molecular Weight
27,010 Da
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for P25427
Species
Rattus norvegicus (Rat)
Tag
C-terminal 10xHis-tagged
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Product Categories/Family for Ngf recombinant protein

Others
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

SDS-PAGE of Ngf recombinant protein

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Rat NGF Recombinant Protein – Cat# MBS9021601

Other Notes

Small volumes of Ngf recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ngf purchase

MBS9021601 is a recombinant protein targeting Ngf. Also known as Recombinant Rat Beta-nerve growth factor (Ngf). Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for Ngf. It may not necessarily be applicable to this product.

NCBI GI #
1352495
NCBI GeneID
310738
NCBI Accession #
P25427.2
UniProt Primary Accession #
P25427
UniProt Related Accession #
P25427
NCBI Official Full Name
Beta-nerve growth factor
NCBI Official Synonym Full Names
nerve growth factor (beta polypeptide)
NCBI Official Symbol
Ngf
NCBI Official Synonym Symbols
Ngfb; beta-NGF
UniProt Protein Name
Beta-nerve growth factor
Protein Family
Beta-nerve growth factor
UniProt Gene Name
Ngf
UniProt Synonym Gene Names
Ngfb; Beta-NGF
UniProt Entry Name
NGF_RAT
NCBI Protein Information
beta-nerve growth factor

UniProt Comments for Ngf

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI.
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Research Articles on Ngf

1. NGF played an important role in the activation of Akt and subsequent up-regulation of BDNF in the sensory neurons in visceral inflammation such as cystitis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Ngf recombinant protein

 Reference Product  PubMed Publications
 Ntrk1 recombinant protein  >2001 publications with Ngf and Ntrk1
 Ntrk2 recombinant protein  >502 publications with Ngf and Ntrk2
 Ngfr recombinant protein  >239 publications with Ngf and Ngfr
 Casp3 recombinant protein  >173 publications with Ngf and Casp3
 Shc1 recombinant protein  >26 publications with Ngf and Shc1
 Mapk8 recombinant protein  >10 publications with Ngf and Mapk8
 Ntf4 recombinant protein  >7 publications with Ngf and Ntf4
 Shc3 recombinant protein  >2 publications with Ngf and Shc3

Pathways associated with Ngf recombinant protein

 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activation Of TRKA Receptors Pathway antibodies  Activation Of TRKA Receptors Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Axonal Growth Stimulation Pathway antibodies  Axonal Growth Stimulation Pathway Diagram
 Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies  Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
 Ceramide Signalling Pathway antibodies  Ceramide Signalling Pathway Diagram
 Frs2-mediated Activation Pathway antibodies  Frs2-mediated Activation Pathway Diagram
 Id Signaling Pathway antibodies  Id Signaling Pathway Diagram
 Inflammatory Mediator Regulation Of TRP Channels Pathway antibodies  Inflammatory Mediator Regulation Of TRP Channels Pathway Diagram

Diseases associated with Ngf recombinant protein

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2164 publications with Ngf and Nervous System Diseases
 Pain Antibodies  >730 publications with Ngf and Pain
 Neurodegenerative Diseases Antibodies  >712 publications with Ngf and Neurodegenerative Diseases
 Inflammation Antibodies  >682 publications with Ngf and Inflammation
 Nerve Degeneration Antibodies  >600 publications with Ngf and Nerve Degeneration
 Peripheral Nervous System Diseases Antibodies  >457 publications with Ngf and Peripheral Nervous System Diseases
 Necrosis Antibodies  >442 publications with Ngf and Necrosis
 Hyperalgesia Antibodies  >309 publications with Ngf and Hyperalgesia
 Heart Diseases Antibodies  >115 publications with Ngf and Heart Diseases
 Neurobehavioral Manifestations Antibodies  >111 publications with Ngf and Neurobehavioral Manifestations

Organs/Tissues associated with Ngf recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >110 publications with Ngf and Connective Tissue
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