Hadv-5 E1B-55K/E1B-495R Recombinant Protein – Cat# MBS1587960

SDS-PAGE for Recombinant Human adenovirus C serotype 5 (HAdV-5)(Human adenovirus 5) E1B-55K protein

E1B-55K/E1B-495R Hadv-5 Recombinant Protein

Name: E1B-55K/E1B-495R Recombinant Protein; Hadv-5; MyBioSource
Brand: MyBioSource
SKU: MBS1587960
Price: 435 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1587960

MBS1587960 is a recombinant protein targeting E1B-55K/E1B-495R. Also known as Recombinant HAdV-5 E1B-55K/E1B-495R Protein, N-GST & C-His. Host: E coli. More details

$435 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

E1B-55K/E1B-495R, Recombinant Protein

Full Product Name

Recombinant HAdV-5 E1B-55K/E1B-495R Protein, N-GST & C-His

Product Gene Name

E1B-55K/E1B-495R recombinant protein

Product Synonym Names

E1B 55 kDa protein; E1B-55K; E1B protein; large T-antigen; E1B-495R

Sequence

Glu2-Gln79

Host

E coli

Form/Format

Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Purity/Purification

>90% as determined by SDS-PAGE.

Preparation and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 degree C for frequent use. Store at -20 to -80 degree C for twelve months from the date of receipt.

Molecular Weight

20,614 Da

3D Structure

ModBase 3D Structure for P03243

Species

Human adenovirus C serotype 5 (HAdV-5)(Human adenovirus 5)

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Product Categories/Family for E1B-55K/E1B-495R recombinant protein

Recombinant Protein; Other Proteins; Proteins

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for E1B-55K/E1B-495R recombinant protein

ELISA (EIA), Immunogen, SDS-PAGE, Western Blot (WB), Bioactivity testing in progress

Other Notes

Small volumes of E1B-55K/E1B-495R recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for E1B-55K/E1B-495R. It may not necessarily be applicable to this product.

NCBI GI #

56160532

NCBI GeneID

NCBI Accession #

AP_000199.1

UniProt Primary Accession #

P03243

UniProt Secondary Accession #

Q64827

GenBank Nucleotide #

AC_000008.1

NCBI Official Full Name

E1B 55K

UniProt Protein Name

E1B 55 kDa protein

UniProt Synonym Protein Names

E1B protein, large T-antigen; E1B-495R

UniProt Gene Name

E1B-55K

UniProt Synonym Gene Names

E1B-55K

UniProt Comments for E1B-55K/E1B-495R

Plays a major role to prevent cellular inhibition of viral genome replication. Assembles an SCF-like E3 ubiquitin ligase complex based on the cellular proteins ELOB, ELOC, CUL5 and RBX1, in cooperation with viral E4orf6. This viral RING-type ligase ubiquitinates cellular substrates and targets them to proteasomal degradation: TP53/p53, LIG4, MRE11-RAD50-NBS1 (MRN) complex, ITGA3, DAXX and BLM. Degradation of host TP53/p53 activity is essential for preventing E1A-induced TP53 accumulation that would otherwise lead to cell apoptosis and growth arrest. E1B-55K also inactivates TP53 transcription-factor activity by binding its transactivation domain. E1B-55K also functions as a SUMO1 E3 ligase for TP53 which causes the latter to be sequestered in promyelocytic leukemia (PML) nuclear bodies thereby contributing to maximal inhibition of TP53 function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with E1B-55K/E1B-495R recombinant protein

Reference Product	PubMed Publications
FLNA recombinant protein	>12 publications with E1B-55K/E1B-495R and FLNA