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Envelope Glycoprotein (Gp), Partial (GP) Crimean-Congo Hemorrhagic Fever Virus Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9019725

Glycoprotein N]: Glycoprotein N and glycoprotein C interact with each other and are present at the surface of the virion (Probable). Glycoprotein N probably locks the Gn-Gc complex in a prefusion state. Glycoprotein N and glycoprotein C are able to attach the… More details

$665 / 0.02 mg (Mammalian-Cell) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Envelope glycoprotein (GP), partial, Recombinant Protein
Full Product Name
Recombinant Crimean-Congo hemorrhagic fever virus Envelope glycoprotein (GP), partial-VLPs
Product Gene Name
Product Synonym Names
M polyprotein; Gn; 37 kDa protein; Glycoprotein G2; NSm; 15 kDa protein; Gc; 75 kDa protein; Glycoprotein G1
Sequence
AVEMENLPAG TWEREEDLTN FCHQECQVTE TECLCPYEAL VLRKPLFLDS TAKGMKNLLN STSLETSLSI EAPWGAINVQ STYKPTVSTA NIALSWSSVE HRGNKILVSG RSESIMKLEE RTGISWDLGV EDASESKLLT VSVMDLSQMY SPVFEYLSGD RQVGEWPKAT CTGDCPERCG CTSSTCLHKE WPHSRNWRCN PTWCWGVGTG CTCCGLDVKD LFTDYMFVKW KVEYIKTEAI VCVELTSQER QCSLIEAGTR FNLGPVTITL SEPRNIQQKL PPEIITLHPR IEEGFFDLMH VQKVLSASTV CKLQSCTHGV PGDLQVYHIG NLLKGDKVNG HLIHKIEPHF NTSWMSWDGC DLDYYCNMGD WPSCTYTGVT QHNHASFVNL LNIETDYTKN FHFHSKRVTA HGDTPQLDLK ARPTYGAGEI TVLVEVADME LHTKKIEISG LKFASLACTG CYACSSGISC KVRIHVDEPD ELTVHVKSDD PDVVAASSSL MARKLEFGTD STFKAFSAMP KTSLCFYIVE REHCKSCSEE DTKKCVNTKL EQPQSILIEH KGTIIGKQNS TCTAKASCWL ESVKSFFYGL KNMLSGIFGN VFMGIFLFLA PFILLILFFM FGWRILFCFK CCRRTRGLFK YRHLKDDEET GYRRIIEKLN NKKGKNKLLD GERLADRRIA ELFSTKTHIG
Host
Mammalian cell
Form/Format
Lyophilized powder
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4.
Purity/Purification
The purity information is not available for VLPs proteins. (lot specific)
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL. Aliquot for long-term storage at -80 degree C.Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
The VLPs are expressed from human 293 cells (HEK293). Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended. Store the protein at -20 degree C/-80 degree C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Molecular Weight
186,589 Da
Sequence Positions
995-1684aa; Partial
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for Q8JSZ3
Species
Crimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970)(CCHFV)
Tag
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Research Area
Signal Transduction
Transmembrane Domain
1TM
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Testing Data of GP recombinant protein

is detected by Mouse anti-6*His monoclonal antibody.
Crimean-Congo Hemorrhagic Fever Virus GP Recombinant Protein – Cat# MBS9019725

Other Notes

Small volumes of GP recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GP purchase

MBS9019725 is a recombinant protein targeting GP. Also known as Recombinant Crimean-Congo hemorrhagic fever virus Envelope glycoprotein (GP), partial-VLPs. Host: Mammalian cell.

Additional Details

NCBI/Uniprot data below describe general gene information for GP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
GenBank Nucleotide #
NCBI Official Full Name
glycoprotein
NCBI Official Symbol
CCFHV_sMgp1
UniProt Protein Name
Envelopment polyprotein
UniProt Synonym Protein Names
Glycoprotein G1
UniProt Gene Name
GP
UniProt Synonym Gene Names
Gc
UniProt Entry Name
GP_CCHFI
NCBI Protein Information
glycoprotein precursor

UniProt Comments for GP

Glycoprotein C and glycoprotein N interact with each other and are present at the surface of the virion. They are able to attach the virion to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promote fusion of viral membrane with host endosomal membrane after endocytosis of the virion ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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