DNA Replication Licensing Factor MCM3 (MCM3) ELISA Kit for Sheep

Name: Sheep MCM3 ELISA Kit; DNA Replication Licensing Factor MCM3; MyBioSource
Brand: MyBioSource
SKU: MBS7266060
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS7266060

This ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the targets. More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

DNA Replication Licensing Factor MCM3, ELISA Kit

Full Product Name

Sheep DNA Replication Licensing Factor MCM3 ELISA Kit

Product Gene Name

MCM3 elisa kit

Species Reactivity

Sheep

Preparation and Storage

Store at 2-8 degree C.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

Molecular Weight

95,908 Da

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Reproducibility

Intra-Assay: CV<10%
Inter-Assay: CV<15%

Reactivity Note

No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of MCM3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCM3 purchase

MBS7266060 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA Replication Licensing Factor MCM3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MCM3. The ELISA analytical biochemical technique of the MBS7266060 kit is based on MCM3 antibody-MCM3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MCM3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MCM3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MCM3. It may not necessarily be applicable to this product.

NCBI GI #

1484505977

NCBI GeneID

4172

NCBI Accession #

NP_001353304.1

UniProt Secondary Accession #

Q92660; Q9BTR3; Q9NUE7; B4DWW4

UniProt Related Accession #

P25205

GenBank Nucleotide #

NM_001366375.1

NCBI Official Full Name

DNA replication licensing factor MCM3 isoform 9

NCBI Official Synonym Full Names

minichromosome maintenance complex component 3

NCBI Official Symbol

MCM3

NCBI Official Synonym Symbols

HCC5; P1.h; RLFB; P1-MCM3

UniProt Protein Name

DNA replication licensing factor MCM3

UniProt Synonym Protein Names

DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102

Protein Family

DNA replication licensing factor

UniProt Gene Name

MCM3

NCBI Protein Information

DNA replication licensing factor MCM3

NCBI Summary for MCM3

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018]

UniProt Comments for MCM3

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

Research Articles on MCM3

1. this study shows that MCM3 is a novel proliferation marker in oral squamous cell carcinoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with MCM3 elisa kit

Products by Pathway	Pathway Diagram
Activation Of ATR In Response To Replication Stress Pathway antibodies	Activation Of ATR In Response To Replication Stress Pathway Diagram
Activation Of The Pre-replicative Complex Pathway antibodies	Activation Of The Pre-replicative Complex Pathway Diagram
Assembly Of The Pre-replicative Complex Pathway antibodies	Assembly Of The Pre-replicative Complex Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Checkpoints Pathway antibodies	Cell Cycle Checkpoints Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
DNA Replication Pathway antibodies	DNA Replication Pathway Diagram