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TEK Cell Lysate – Tissue Lysate

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8117025

TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation,… More details

$315 / 0.3 mg +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TIE2 (TEK), Cell Lysate
Full Product Name
Mouse TIE2 HEK293 Overexpression Lysate
Product Gene Name
TEK cell lysate
Product Synonym Names
Mouse Tie2/TEK HEK293 Cell Lysate (WB positive control); TEK tyrosine kinase, endothelial; Mouse AA517024 Overexpression Lysate; Mouse Cd202b Overexpression Lysate; Mouse Hyk Overexpression Lysate; Mouse STK1 Overexpression Lysate; Mouse Tie-2 Overexpression Lysate; Mouse Tie2 Overexpression Lysate
Sequence
Met1-Lys74 4
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Molecular Weight
125,701 Da
Sequence Positions
737aa
3D Structure
ModBase 3D Structure for Q02858
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse TEK (Q02858) (Met1-Lys744) was expressed with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Product Categories/Family for TEK cell lysate

Receptor Tyrosine Kinases (RTKs) Cancer Drug Targets Hemangioblast Markers
Applications Tested/Suitable for TEK cell lysate
Western Blot (WB)
Application Notes for TEK cell lysate
WB: Use at an assay dependent dilution.

Product References and Citations for TEK cell lysate

Jones N, et al. (1998) The Tek/Tie2 receptor signals through a novel Dok-related docking protein, Dok-R. Oncogene. 17(9): 1097-108.Sato A, et al. (1998) Characterization of TEK receptor tyrosine kinase and its ligands, Angiopoietins, in human hematopoietic progenitor cells. Int Immunol. 10(8): 1217-27.Huang L, et al. (1995) GRB2 and SH-PTP2: potentially important endothelial signaling molecules downstream of the TEK/TIE2 receptor tyrosine kinase. Oncogene. 11(10): 2097-103.

Other Notes

Small volumes of TEK cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TEK purchase

MBS8117025 is a cell lysate targeting TEK. Also known as Mouse TIE2 HEK293 Overexpression Lysate. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for TEK. It may not necessarily be applicable to this product.

NCBI GI #
594542525
NCBI GeneID
21687
NCBI Accession #
NP_001277478.1
UniProt Primary Accession #
Q02858
UniProt Related Accession #
Q02858
GenBank Nucleotide #
NM_001290549.1
NCBI Official Full Name
angiopoietin-1 receptor isoform 2
NCBI Official Synonym Full Names
endothelial-specific receptor tyrosine kinase
NCBI Official Symbol
Tek
NCBI Official Synonym Symbols
Hyk; STK1; Tie2; Tie-2; Cd202b; AA517024
UniProt Protein Name
Angiopoietin-1 receptor
UniProt Synonym Protein Names
Endothelial tyrosine kinase; HYK; STK1; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; mTIE2; p140 TEK; CD_antigen: CD202b
Protein Family
Angiopoietin-1 receptor
UniProt Gene Name
Tek
UniProt Synonym Gene Names
Hyk; Tie-2; Tie2; mTIE2
UniProt Entry Name
TIE2_MOUSE
NCBI Protein Information
angiopoietin-1 receptor

UniProt Comments for TEK

TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Tie family

Cellular Component: actin filament; apical plasma membrane; basal plasma membrane; basolateral plasma membrane; cell surface; integral to membrane; integral to plasma membrane; intercellular junction; lipid raft; microvillus; perinuclear region of cytoplasm; stress fiber

Molecular Function: growth factor binding; protein binding; protein-tyrosine kinase activity; receptor activity

Biological Process: angiogenesis; cell-cell adhesion; cell-matrix adhesion; endothelial cell proliferation; heart development; hemopoiesis; negative regulation of angiogenesis; negative regulation of apoptosis; patterning of blood vessels; peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; positive regulation of cell adhesion; positive regulation of cytokine secretion during immune response; positive regulation of focal adhesion formation; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of protein import into nucleus; positive regulation of protein kinase B signaling cascade; positive regulation of vascular endothelial growth factor receptor signaling pathway; protein amino acid autophosphorylation; protein oligomerization; regulation of angiogenesis; regulation of cell migration; regulation of endothelial cell proliferation; regulation of establishment and/or maintenance of cell polarity; response to estrogen stimulus; response to retinoic acid; sprouting angiogenesis; Tie receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; vasculogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TEK cell lysate

 Reference Product  PubMed Publications
 Angpt2 cell lysate  >52 publications with TEK and Angpt2
 Angpt1 cell lysate  >44 publications with TEK and Angpt1
 Vegfa cell lysate  >18 publications with TEK and Vegfa
 Grb7 cell lysate  >3 publications with TEK and Grb7
 Dok2 cell lysate  >3 publications with TEK and Dok2
 Grb2 cell lysate  >2 publications with TEK and Grb2
 Ptpn11 cell lysate  >2 publications with TEK and Ptpn11
 Pik3r1 cell lysate  >1 publications with TEK and Pik3r1
 Angpt4 cell lysate  >1 publications with TEK and Angpt4
 Shc1 cell lysate  >1 publications with TEK and Shc1

Pathways associated with TEK cell lysate

 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Of Activated FGFR1 Pathway antibodies  Downstream Signaling Of Activated FGFR1 Pathway Diagram
 Downstream Signaling Of Activated FGFR2 Pathway antibodies  Downstream Signaling Of Activated FGFR2 Pathway Diagram

Diseases associated with TEK cell lysate

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >72 publications with TEK and Cardiovascular Diseases
 Neovascularization, Pathologic Antibodies  >59 publications with TEK and Neovascularization, Pathologic
 Inflammation Antibodies  >54 publications with TEK and Inflammation
 Brain Diseases Antibodies  >25 publications with TEK and Brain Diseases
 Heart Diseases Antibodies  >24 publications with TEK and Heart Diseases
 Neoplasms, Experimental Antibodies  >23 publications with TEK and Neoplasms, Experimental
 Hypertension Antibodies  >18 publications with TEK and Hypertension
 Liver Diseases Antibodies  >17 publications with TEK and Liver Diseases
 Necrosis Antibodies  >16 publications with TEK and Necrosis
 Pain Antibodies  >15 publications with TEK and Pain

Organs/Tissues associated with TEK cell lysate

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >68 publications with TEK and Muscle
 Embryonic Tissue Antibodies  >65 publications with TEK and Embryonic Tissue
 Lung Antibodies  >60 publications with TEK and Lung
 Heart Antibodies  >50 publications with TEK and Heart
 Brain Antibodies  >41 publications with TEK and Brain
 Kidney Antibodies  >30 publications with TEK and Kidney
 Skin Antibodies  >28 publications with TEK and Skin
 Eye Antibodies  >15 publications with TEK and Eye
 Ovary Antibodies  >8 publications with TEK and Ovary
 Spleen Antibodies  >7 publications with TEK and Spleen
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