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AKR1E2 siRNA – 1,5-Anhydro-D-Fructose Reductase Isoform 1 (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8239307

MBS8239307 is an siRNA targeting AKR1E2. Also known as AKR1E2 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit AKR1E2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AKR1E2, siRNA
Full Product Name
AKR1E2 siRNA (Human)
Product Gene Name
Product Synonym Names
AKR1CL2; AKRDC1; 1.5-anhydro-D-fructose reductase; AF reductase; Aldo-keto reductase family 1 member C-like protein 2; Aldo-keto reductase family 1 member E2; LoopADR; Testis-specific protein; hTSP
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
AKR1E2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
25,693 Da
3D Structure
ModBase 3D Structure for Q96JD6
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AKR1E2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for AKR1E2 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of AKR1E2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AKR1E2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
1,5-anhydro-D-fructose reductase isoform 1
NCBI Official Synonym Full Names
aldo-keto reductase family 1, member E2
NCBI Official Symbol
AKR1E2
NCBI Official Synonym Symbols
hTSP; htAKR; AKRDC1; AKR1CL2; LoopADR
UniProt Protein Name
1,5-anhydro-D-fructose reductase
UniProt Synonym Protein Names
Aldo-keto reductase family 1 member C-like protein 2; Aldo-keto reductase family 1 member E2; LoopADR; Testis-specific protein; hTSP
UniProt Gene Name
AKR1E2
UniProt Synonym Gene Names
AKR1CL2; AKRDC1; AF reductase; hTSP
UniProt Entry Name
AKCL2_HUMAN
NCBI Protein Information
1,5-anhydro-D-fructose reductase

NCBI Summary for AKR1E2

The protein encoded by this gene is a member of the aldo-keto reductase superfamily. Members in this family are characterized by their structure (evolutionarily highly conserved TIM barrel) and function (NAD(P)H-dependent oxido-reduction of carbonyl groups). Transcripts of this gene have been reported in specimens of human testis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
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UniProt Comments for AKR1E2

AKR1CL2: Catalyzes the NADPH-dependent reduction of 1,5-anhydro- D-fructose (AF) to 1,5-anhydro-D-glucitol. Can also catalyze the reduction of various aldehydes and quinones. Has low NADPH-dependent reductase activity towards 9,10- phenanthrenequinone (in vitro). Belongs to the aldo/keto reductase family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.263; Oxidoreductase

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: cytoplasm

Molecular Function: 1,5-anhydro-D-fructose reductase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with AKR1E2 sirna

 Reference Product  PubMed Publications
 MFSD6L sirna  >1 publications with AKR1E2 and MFSD6L

Organs/Tissues associated with AKR1E2 sirna

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