GABRA6 siRNA – Gamma-Aminobutyric Acid Receptor Subunit Alpha-6 (Human)

Name: GABRA6 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8210060
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8210060

MBS8210060 is an siRNA targeting GABRA6. Also known as GABRA6 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit GABRA6 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GABRA6, siRNA

Full Product Name

GABRA6 siRNA (Human)

Product Gene Name

GABRA6 sirna

Product Synonym Names

Gamma-aminobutyric acid receptor subunit alpha-6; GABA(A) receptor subunit alpha-6

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

GABRA6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

51,024 Da

OMIM

137143

3D Structure

ModBase 3D Structure for Q16445

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GABRA6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for GABRA6 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of GABRA6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GABRA6. It may not necessarily be applicable to this product.

NCBI GI #

157389018

NCBI GeneID

2559

NCBI Accession #

NP_000802.2

UniProt Primary Accession #

Q16445

UniProt Secondary Accession #

Q4VAV2; A8K096

UniProt Related Accession #

Q16445

GenBank Nucleotide #

NM_000811.2

NCBI Official Full Name

gamma-aminobutyric acid receptor subunit alpha-6

NCBI Official Synonym Full Names

gamma-aminobutyric acid (GABA) A receptor, alpha 6

NCBI Official Symbol

GABRA6

UniProt Protein Name

Gamma-aminobutyric acid receptor subunit alpha-6

UniProt Synonym Protein Names

GABA(A) receptor subunit alpha-6

Protein Family

Gamma-aminobutyric acid receptor

UniProt Gene Name

GABRA6

UniProt Entry Name

GBRA6_HUMAN

NCBI Protein Information

gamma-aminobutyric acid receptor subunit alpha-6

NCBI Summary for GABRA6

GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. [provided by RefSeq, Jul 2008]

UniProt Comments for GABRA6

GABRA6: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA6 sub-subfamily.

Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral; Channel, chloride; Transporter, ion channel

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction

Molecular Function: chloride channel activity; GABA-A receptor activity; benzodiazepine receptor activity; extracellular ligand-gated ion channel activity

Biological Process: synaptic transmission; transport; signal transduction; transmembrane transport; gamma-aminobutyric acid signaling pathway

Research Articles on GABRA6

1. The gene-gene interaction at two loci involving GABRA6 and Synapsin II revealed a significant association for development of idiopathic generalized epilepsy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GABRA6 sirna

Reference Product	PubMed Publications
GABRA1 sirna	>25 publications with GABRA6 and GABRA1
GABRG2 sirna	>19 publications with GABRA6 and GABRG2
GABRB2 sirna	>18 publications with GABRA6 and GABRB2
GABRA2 sirna	>7 publications with GABRA6 and GABRA2
GABRB3 sirna	>7 publications with GABRA6 and GABRB3
GABRB1 sirna	>3 publications with GABRA6 and GABRB1
GABRG3 sirna	>2 publications with GABRA6 and GABRG3

Pathways associated with GABRA6 sirna

Products by Pathway	Pathway Diagram
GABA A Receptor Activation Pathway antibodies	GABA A Receptor Activation Pathway Diagram
GABA Receptor Activation Pathway antibodies	GABA Receptor Activation Pathway Diagram
GABAergic Synapse Pathway antibodies	GABAergic Synapse Pathway Diagram
GABAergic Synapse Pathway antibodies	GABAergic Synapse Pathway Diagram
Ion Channel Transport Pathway antibodies	Ion Channel Transport Pathway Diagram
Ligand-gated Ion Channel Transport Pathway antibodies	Ligand-gated Ion Channel Transport Pathway Diagram
Morphine Addiction Pathway antibodies	Morphine Addiction Pathway Diagram
Morphine Addiction Pathway antibodies	Morphine Addiction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with GABRA6 sirna

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>19 publications with GABRA6 and Nervous System Diseases
Brain Diseases Antibodies	>17 publications with GABRA6 and Brain Diseases
Central Nervous System Diseases Antibodies	>14 publications with GABRA6 and Central Nervous System Diseases
Epilepsy Antibodies	>13 publications with GABRA6 and Epilepsy
Seizures Antibodies	>9 publications with GABRA6 and Seizures
Neurologic Manifestations Antibodies	>8 publications with GABRA6 and Neurologic Manifestations
Anxiety Disorders Antibodies	>7 publications with GABRA6 and Anxiety Disorders
Schizophrenia Antibodies	>4 publications with GABRA6 and Schizophrenia
Pain Antibodies	>3 publications with GABRA6 and Pain
Ataxia Antibodies	>3 publications with GABRA6 and Ataxia

Organs/Tissues associated with GABRA6 sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>51 publications with GABRA6 and Brain
Muscle Antibodies	>2 publications with GABRA6 and Muscle