NSMCE2 siRNA – E3 SUMO-Protein Ligase NSE2 (Human)

NSMCE2: E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, and maybe the cohesin components RAD21 and STAG2. Required for recruitment of telomeres to PML nuclear bodies. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines. Required for sister chromatid cohesion during prometaphase and mitotic progression. Belongs to the NSE2 family.

Protein type: EC 6.3.2.-; SUMO conjugating system; EC 6.-.-.-; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: chromosome, telomeric region; PML body; nucleus

Molecular Function: protein binding; zinc ion binding; SUMO ligase activity; ligase activity

Biological Process: mitosis; protein sumoylation; positive regulation of maintenance of mitotic sister chromatid cohesion; cell division; telomere maintenance via recombination; positive regulation of mitotic metaphase/anaphase transition; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining