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PLCD3 siRNA – 1-Phosphatidylinositol 4,5-Bisphosphate Phosphodiesterase Delta-3 (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8211674

MBS8211674 is an siRNA targeting PLCD3. Also known as PLCD3 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit PLCD3 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PLCD3, siRNA
Full Product Name
PLCD3 siRNA (Human)
Product Gene Name
PLCD3 sirna
Product Synonym Names
KIAA1964; 1-phosphatidylinositol 4.5-bisphosphate phosphodiesterase delta-3; Phosphoinositide phospholipase C-delta-3; Phospholipase C-delta-3; PLC-delta-3
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
PLCD3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
89,258 Da
OMIM
608795
3D Structure
ModBase 3D Structure for Q8N3E9
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PLCD3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for PLCD3 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of PLCD3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PLCD3. It may not necessarily be applicable to this product.

NCBI GI #
19115964
NCBI GeneID
113026
NCBI Accession #
NP_588614.1
UniProt Primary Accession #
Q8N3E9
UniProt Secondary Accession #
Q8TEC1; Q8TF37; Q96FL6
UniProt Related Accession #
Q8N3E9
GenBank Nucleotide #
NM_133373.4
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3
NCBI Official Synonym Full Names
phospholipase C, delta 3
NCBI Official Symbol
PLCD3
NCBI Official Synonym Symbols
PLC-delta-3
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3
UniProt Synonym Protein Names
Phosphoinositide phospholipase C-delta-3; Phospholipase C-delta-3; PLC-delta-3
Protein Family
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
UniProt Gene Name
PLCD3
UniProt Synonym Gene Names
KIAA1964; PLC-delta-3
UniProt Entry Name
PLCD3_HUMAN
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3

NCBI Summary for PLCD3

This gene encodes a member of the phospholipase C family, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine, sphingosine, and several phospholipids. [provided by RefSeq, Jul 2008]
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UniProt Comments for PLCD3

PLCD3: Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Essential for trophoblast and placental development. May participate in cytokinesis by hydrolyzing PIP2 at the cleavage furrow.

Protein type: EC 3.1.4.11; Carbohydrate Metabolism - inositol phosphate; Phospholipase

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: plasma membrane; cytosol; cleavage furrow

Molecular Function: signal transducer activity; metal ion binding; phosphoinositide phospholipase C activity

Biological Process: inositol phosphate metabolic process; angiogenesis; lipid catabolic process; regulation of cell proliferation
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Research Articles on PLCD3

1. PLCdelta3 regulates microvilli formation in enterocytes and the radial migration of neurons in the cerebral cortex of the developing brain. [review]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PLCD3 sirna

 Reference Product  PubMed Publications
 ITPR3 sirna  >1 publications with PLCD3 and ITPR3

Pathways associated with PLCD3 sirna

 Products by Pathway  Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway Diagram
 D-myo-inositol-5-phosphate Metabolism Pathway antibodies  D-myo-inositol-5-phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway antibodies  Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway Diagram
 Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway antibodies  Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram

Diseases associated with PLCD3 sirna

 Disease Name  Pubmed Publications
 Hypertension Antibodies  >5 publications with PLCD3 and Hypertension
 Weight Loss Antibodies  >2 publications with PLCD3 and Weight Loss
 Cardiomegaly Antibodies  >1 publications with PLCD3 and Cardiomegaly
 Fibrosis Antibodies  >1 publications with PLCD3 and Fibrosis
 Breast Neoplasms Antibodies  >1 publications with PLCD3 and Breast Neoplasms
 Disease Models, Animal Antibodies  >1 publications with PLCD3 and Disease Models, Animal
 Hypertrophy Antibodies  >1 publications with PLCD3 and Hypertrophy

Organs/Tissues associated with PLCD3 sirna

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with PLCD3 and Blood
 Embryonic Tissue Antibodies  >3 publications with PLCD3 and Embryonic Tissue
 Heart Antibodies  >3 publications with PLCD3 and Heart
 Muscle Antibodies  >2 publications with PLCD3 and Muscle
 Liver Antibodies  >2 publications with PLCD3 and Liver
 Brain Antibodies  >2 publications with PLCD3 and Brain
 Connective Tissue Antibodies  >1 publications with PLCD3 and Connective Tissue
 Skin Antibodies  >1 publications with PLCD3 and Skin
 Intestine Antibodies  >1 publications with PLCD3 and Intestine
 Placenta Antibodies  >1 publications with PLCD3 and Placenta
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