• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

RAPH1 siRNA – Ras-Associated and Pleckstrin Homology Domains-Containing Protein 1 Isoform 3 (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8232181

MBS8232181 is an siRNA targeting RAPH1. Also known as RAPH1 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit RAPH1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RAPH1, siRNA
Full Product Name
RAPH1 siRNA (Human)
Product Gene Name
Product Synonym Names
ALS2CR18; ALS2CR9; KIAA1681; LPD; PREL2; RMO1; Ras-associated and pleckstrin homology domains-containing protein 1; RAPH1; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein; Lamellipodin; Proline-rich EVH1 ligand 2; PREL-2; Protein RMO1
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
RAPH1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
72,874 Da
OMIM
609035
3D Structure
ModBase 3D Structure for Q70E73
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RAPH1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for RAPH1 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of RAPH1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RAPH1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ras-associated and pleckstrin homology domains-containing protein 1 isoform 3
NCBI Official Synonym Full Names
Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
NCBI Official Symbol
RAPH1
NCBI Official Synonym Symbols
LPD; RMO1; PREL2; PREL-2; ALS2CR9; ALS2CR18; RalGDS/AF-6
UniProt Protein Name
Ras-associated and pleckstrin homology domains-containing protein 1
UniProt Synonym Protein Names
Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein; Lamellipodin; Proline-rich EVH1 ligand 2; PREL-2; Protein RMO1
UniProt Gene Name
RAPH1
UniProt Synonym Gene Names
ALS2CR18; ALS2CR9; KIAA1681; LPD; PREL2; RMO1; RAPH1; PREL-2
UniProt Entry Name
RAPH1_HUMAN
NCBI Protein Information
ras-associated and pleckstrin homology domains-containing protein 1

UniProt Comments for RAPH1

RAPH1: Mediator of localized membrane signals. Implicated in the regulation of lamellipodial dynamics. Negatively regulates cell adhesion. Belongs to the MRL family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: cytoskeleton; lamellipodium; cytoplasm; plasma membrane; filopodium

Molecular Function: protein binding

Biological Process: axon extension; cell-matrix adhesion; signal transduction
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000