TSPAN12 siRNA – Tetraspanin-12 (Human)

Name: TSPAN12 siRNA; Tetraspanin-12; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8204158
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8204158

MBS8204158 is an siRNA targeting TSPAN12. Also known as TSPAN12 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit TSPAN12 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TSPAN12, siRNA

Full Product Name

TSPAN12 siRNA (Human)

Product Gene Name

TSPAN12 sirna

Product Synonym Names

NET2; TM4SF12; Tetraspanin-12; Tspan-12; Tetraspan NET-2; Transmembrane 4 superfamily member 12

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

TSPAN12 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

22,511 Da

OMIM

613138

3D Structure

ModBase 3D Structure for O95859

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TSPAN12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for TSPAN12 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of TSPAN12 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TSPAN12. It may not necessarily be applicable to this product.

NCBI GI #

6912528

NCBI GeneID

23554

NCBI Accession #

NP_036470.1

UniProt Primary Accession #

O95859

UniProt Secondary Accession #

Q549U9; Q8N5Y0; A4D0V8; B4DRG6

UniProt Related Accession #

O95859

GenBank Nucleotide #

NM_012338.3

NCBI Official Full Name

tetraspanin-12

NCBI Official Synonym Full Names

tetraspanin 12

NCBI Official Symbol

TSPAN12

NCBI Official Synonym Symbols

EVR5; NET2; NET-2; TM4SF12

UniProt Protein Name

Tetraspanin-12

UniProt Synonym Protein Names

Tetraspan NET-2; Transmembrane 4 superfamily member 12

UniProt Gene Name

TSPAN12

UniProt Synonym Gene Names

NET2; TM4SF12; Tspan-12

UniProt Entry Name

TSN12_HUMAN

NCBI Protein Information

tetraspanin-12

NCBI Summary for TSPAN12

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. [provided by RefSeq, Jul 2008]

UniProt Comments for TSPAN12

TSPAN12: a multi-pass membrane protein. Member of the transmembrane 4 superfamily, also known as the tetraspanin family. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1). Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity. Defects in TSPAN12 are the cause of vitreoretinopathy exudative type 5 (EVR5). It is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. Twp isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, misc.

Chromosomal Location of Human Ortholog: 7q31.31

Cellular Component: membrane; integral to plasma membrane; integral to membrane

Molecular Function: Wnt receptor activity

Biological Process: cell surface receptor linked signal transduction; angiogenesis; regulation of angiogenesis

Disease: Exudative Vitreoretinopathy 5

Research Articles on TSPAN12

1. Here we describe a case of a female infant affected by cystic fibrosis and by a severe form of exudative vitreoretinopathy. In particular, we have detected the homozygous missense mutation c.668 T > C in TSPAN12.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TSPAN12 sirna

Reference Product	PubMed Publications
FZD4 sirna	>24 publications with TSPAN12 and FZD4
LRP5 sirna	>23 publications with TSPAN12 and LRP5
NDP sirna	>22 publications with TSPAN12 and NDP
ADAM10 sirna	>2 publications with TSPAN12 and ADAM10
CADPS2 sirna	>1 publications with TSPAN12 and CADPS2

Diseases associated with TSPAN12 sirna

Disease Name	Pubmed Publications
Exudative Vitreoretinopathy 5 Antibodies	>10 publications with TSPAN12 and Exudative Vitreoretinopathy 5
Vascular Diseases Antibodies	>8 publications with TSPAN12 and Vascular Diseases
Disease Susceptibility Antibodies	>5 publications with TSPAN12 and Disease Susceptibility
Cardiovascular Diseases Antibodies	>4 publications with TSPAN12 and Cardiovascular Diseases
Inflammation Antibodies	>3 publications with TSPAN12 and Inflammation
Breast Neoplasms Antibodies	>2 publications with TSPAN12 and Breast Neoplasms
Neoplasm Metastasis Antibodies	>2 publications with TSPAN12 and Neoplasm Metastasis
Hemorrhage Antibodies	>1 publications with TSPAN12 and Hemorrhage
Poisoning Antibodies	>1 publications with TSPAN12 and Poisoning
Ovarian Neoplasms Antibodies	>1 publications with TSPAN12 and Ovarian Neoplasms

Organs/Tissues associated with TSPAN12 sirna

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>19 publications with TSPAN12 and Eye
Vascular Antibodies	>11 publications with TSPAN12 and Vascular
Brain Antibodies	>6 publications with TSPAN12 and Brain
Bone Antibodies	>3 publications with TSPAN12 and Bone
Muscle Antibodies	>2 publications with TSPAN12 and Muscle
Heart Antibodies	>2 publications with TSPAN12 and Heart
Lung Antibodies	>2 publications with TSPAN12 and Lung
Ascites Antibodies	>1 publications with TSPAN12 and Ascites
Liver Antibodies	>1 publications with TSPAN12 and Liver
Adrenal Gland Antibodies	>1 publications with TSPAN12 and Adrenal Gland