• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

F4/80 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS216408

MBS216408 is an antibody targeting F4/80. Also known as RAT ANTI MOUSE F4/80:APC. Host: Rat. More details

$670 / 100 Tests +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
F4/80, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE F4/80:APC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
Cl:A3-1
Host
Rat
Form/Format
APC
Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Molecular Weight
102,130 Da
Reconstitution
Reconstitute with 1 ml distilled water
Perservative Stabilisers||0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Preparation||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-F4/80 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-F4/80 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Testing Data of anti-F4/80 antibody

Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC (MBS216414)
Rat Anti-F4/80 Monoclonal Antibody – Cat# MBS216408

Testing Data of anti-F4/80 antibody

Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 (MBS216424), red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS216408

Testing Data of anti-F4/80 antibody

Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS216408

Testing Data of anti-F4/80 antibody

Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750 (MBS219371P750)
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS216408

Testing Data of anti-F4/80 antibody

Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin (MBS216412)
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS216408

Testing Data of anti-F4/80 antibody

Frozen mouse spleen stained with A: Rat anti Mouse F4/80 (MBS219371) followed by Goat anti Rat IgG:HRP (MBS235195) or B: Rat anti Mouse F4/80 (MBS219371) preincubated with 2 molar excess of Human anti Idiotypic MBS219371 (HCA154) followed by Goat anti Rat IgG:HRP (MBS235195)
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS216408

Testing Data of anti-F4/80 antibody

Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin (MBS216409)
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS216408

Testing Data of anti-F4/80 antibody

Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue(MBS219371PB)
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS216408

Testing Data of anti-F4/80 antibody

Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS216408

Testing Data of anti-F4/80 antibody

Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin (MBS216410)
Rat Anti-F4/80 Monoclonal Antibody, Testing Data – Cat# MBS216408

Other Notes

Small volumes of anti-F4/80 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for F4/80. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
EGF-like module-containing mucin-like hormone receptor-like 1
NCBI Official Synonym Full Names
EGF-like module containing, mucin-like, hormone receptor-like sequence 1
NCBI Official Symbol
Emr1
NCBI Official Synonym Symbols
Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
UniProt Protein Name
EGF-like module-containing mucin-like hormone receptor-like 1
UniProt Synonym Protein Names
Cell surface glycoprotein F4/80; EGF-like module receptor 1; EMR1 hormone receptor
UniProt Gene Name
Emr1
UniProt Synonym Gene Names
Gpf480
UniProt Entry Name
EMR1_MOUSE
NCBI Protein Information
EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; cell surface glycoprotein F4/80; lymphocyte antigen 71

UniProt Comments for F4/80

EMR1: Could be involved in cell-cell interactions. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 2

Cellular Component: membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; signal transducer activity; transmembrane receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; signal transduction
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000