ACOT4 siRNA – Acyl-Coenzyme A Thioesterase 4 (Mouse)

Name: ACOT4 siRNA; Acyl-Coenzyme A Thioesterase 4; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8221907
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8221907

MBS8221907 is an siRNA targeting ACOT4. Also known as ACOT4 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit ACOT4 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ACOT4, siRNA

Full Product Name

ACOT4 siRNA (Mouse)

Product Gene Name

ACOT4 sirna

Product Synonym Names

PTE1B; PTE2B; Acyl-coenzyme A thioesterase 4; Acyl-CoA thioesterase 4; PTE-2b; Peroxisomal acyl coenzyme A thioester hydrolase Ib; Peroxisomal long-chain acyl-CoA thioesterase Ib; PTE-Ib

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

ACOT4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

46,481 Da

3D Structure

ModBase 3D Structure for Q8BWN8

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ACOT4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for ACOT4 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of ACOT4 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ACOT4. It may not necessarily be applicable to this product.

NCBI GI #

269308227

NCBI GeneID

171282

NCBI Accession #

NP_599008.3

UniProt Primary Accession #

Q8BWN8

UniProt Secondary Accession #

Q8BJQ1; Q8BL20; Q9QYR8

UniProt Related Accession #

Q8BWN8

GenBank Nucleotide #

NM_134247.3

NCBI Official Full Name

acyl-coenzyme A thioesterase 4

NCBI Official Synonym Full Names

acyl-CoA thioesterase 4

NCBI Official Symbol

Acot4

NCBI Official Synonym Symbols

Pte1b; Pte2b; PTE-Ib; B430212I04Rik

UniProt Protein Name

Acyl-coenzyme A thioesterase 4

UniProt Synonym Protein Names

PTE-2b; Peroxisomal acyl coenzyme A thioester hydrolase Ib; Peroxisomal long-chain acyl-CoA thioesterase Ib; PTE-Ib

Protein Family

Acyl-coenzyme A thioesterase

UniProt Gene Name

Acot4

UniProt Synonym Gene Names

Pte1b; Pte2b; Acyl-CoA thioesterase 4; PTE-Ib

UniProt Entry Name

ACOT4_MOUSE

NCBI Protein Information

acyl-coenzyme A thioesterase 4

UniProt Comments for ACOT4

ACOT4: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Succinyl-CoA thioesterase that also hydrolyzes long chain saturated and unsaturated monocarboxylic acyl-CoAs. Belongs to the C/M/P thioester hydrolase family.

Protein type: Lipid Metabolism - unsaturated fatty acid biosynthesis; Hydrolase; EC 3.1.2.2

Cellular Component: peroxisome

Molecular Function: acyl-CoA hydrolase activity; hydrolase activity; thiolester hydrolase activity; succinyl-CoA hydrolase activity; palmitoyl-CoA hydrolase activity; receptor binding

Biological Process: dicarboxylic acid metabolic process; acyl-CoA metabolic process; short-chain fatty acid metabolic process; succinyl-CoA metabolic process; very-long-chain fatty acid metabolic process; saturated monocarboxylic acid metabolic process; long-chain fatty acid metabolic process; unsaturated monocarboxylic acid metabolic process; dicarboxylic acid catabolic process

Research Articles on ACOT4

1. ACOT4 and ACOT8 are responsible for the termination of beta-oxidation of dicarboxylic acids of medium-chain length with the concomitant release of the corresponding free acids

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ACOT4 sirna

Reference Product	PubMed Publications
Acot8 sirna	>2 publications with ACOT4 and Acot8
Acot3 sirna	>2 publications with ACOT4 and Acot3
Cyp4a14 sirna	>1 publications with ACOT4 and Cyp4a14
Cyp4a10 sirna	>1 publications with ACOT4 and Cyp4a10

Pathways associated with ACOT4 sirna

Products by Pathway	Pathway Diagram
Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies	Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies	Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
Fatty Acid Elongation Pathway antibodies	Fatty Acid Elongation Pathway Diagram
Fatty Acid Elongation Pathway antibodies	Fatty Acid Elongation Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Acyl-CoA Hydrolysis Pathway antibodies	Acyl-CoA Hydrolysis Pathway Diagram

Diseases associated with ACOT4 sirna

Disease Name	Pubmed Publications
Fatty Liver Antibodies	>2 publications with ACOT4 and Fatty Liver
Liver Diseases Antibodies	>1 publications with ACOT4 and Liver Diseases

Organs/Tissues associated with ACOT4 sirna

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>3 publications with ACOT4 and Liver
Adipose Tissue Antibodies	>1 publications with ACOT4 and Adipose Tissue
Kidney Antibodies	>1 publications with ACOT4 and Kidney