ADAM18 siRNA – Disintegrin and Metalloproteinase Domain-Containing Protein 18 (Mouse)

Name: ADAM18 siRNA; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8206904
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8206904

MBS8206904 is an siRNA targeting ADAM18. Also known as ADAM18 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit ADAM18 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ADAM18, siRNA

Full Product Name

ADAM18 siRNA (Mouse)

Product Gene Name

ADAM18 sirna

Product Synonym Names

ADAM27; DTGN3; TMDC3; Disintegrin and metalloproteinase domain-containing protein 18; ADAM 18; Disintegrin and metalloproteinase domain-containing protein 27; ADAM 27; Transmembrane metalloproteinase-like. disintegrin-like. and cysteine-rich protein III; tMDC III

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

ADAM18 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

79,210 Da

3D Structure

ModBase 3D Structure for Q9R157

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ADAM18 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for ADAM18 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of ADAM18 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ADAM18. It may not necessarily be applicable to this product.

NCBI GI #

242332494

NCBI GeneID

13524

NCBI Accession #

NP_034214.2

UniProt Primary Accession #

Q9R157

UniProt Secondary Accession #

Q60621; Q80Y08

UniProt Related Accession #

Q9R157

GenBank Nucleotide #

NM_010084.2

NCBI Official Full Name

disintegrin and metalloproteinase domain-containing protein 18

NCBI Official Synonym Full Names

a disintegrin and metallopeptidase domain 18

NCBI Official Symbol

Adam18

NCBI Official Synonym Symbols

Dtgn3; Tmdc3; Adam27; tMDCIII

UniProt Protein Name

Disintegrin and metalloproteinase domain-containing protein 18

UniProt Synonym Protein Names

Disintegrin and metalloproteinase domain-containing protein 27; ADAM 27; Transmembrane metalloproteinase-like, disintegrin-like, and cysteine-rich protein III; tMDC III

Protein Family

Disintegrin and metalloproteinase domain-containing protein

UniProt Gene Name

Adam18

UniProt Synonym Gene Names

Adam27; Dtgn3; Tmdc3; ADAM 18; ADAM 27; tMDC III

UniProt Entry Name

ADA18_MOUSE

NCBI Protein Information

disintegrin and metalloproteinase domain-containing protein 18

UniProt Comments for ADAM18

ADAM18: Sperm surface membrane protein that may be involved in spermatogenesis and fertilization. This is a non catalytic metalloprotease-like protein. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Cellular Component: membrane; integral to membrane

Molecular Function: integrin binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity

Biological Process: multicellular organismal development; spermatogenesis; proteolysis; cell differentiation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ADAM18 sirna

Reference Product	PubMed Publications
Adam7 sirna	>3 publications with ADAM18 and Adam7

Diseases associated with ADAM18 sirna

Disease Name	Pubmed Publications
Infertility, Male Antibodies	>1 publications with ADAM18 and Infertility, Male

Organs/Tissues associated with ADAM18 sirna

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>6 publications with ADAM18 and Testis