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OPLAH siRNA – 5-Oxoprolinase (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8220286

MBS8220286 is an siRNA targeting OPLAH. Also known as OPLAH siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit OPLAH expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
OPLAH, siRNA
Full Product Name
OPLAH siRNA (Mouse)
Product Gene Name
OPLAH sirna
Product Synonym Names
5-oxoprolinase; 5-oxo-L-prolinase; 5-OPase; Pyroglutamase
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
OPLAH siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
137,611 Da
3D Structure
ModBase 3D Structure for Q8K010
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse OPLAH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for OPLAH sirna
RNA Interference (RNAi)

Other Notes

Small volumes of OPLAH sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OPLAH. It may not necessarily be applicable to this product.

NCBI GI #
23346557
NCBI GeneID
75475
NCBI Accession #
NP_694762.1
UniProt Primary Accession #
Q8K010
UniProt Secondary Accession #
Q8R3K2
UniProt Related Accession #
Q8K010
GenBank Nucleotide #
NM_153122.2
NCBI Official Full Name
5-oxoprolinase
NCBI Official Synonym Full Names
5-oxoprolinase (ATP-hydrolysing)
NCBI Official Symbol
Oplah
NCBI Official Synonym Symbols
1700010G02Rik
UniProt Protein Name
5-oxoprolinase
UniProt Synonym Protein Names
5-oxo-L-prolinase; 5-OPase; Pyroglutamase
UniProt Gene Name
Oplah
UniProt Synonym Gene Names
5-OPase
UniProt Entry Name
OPLA_MOUSE
NCBI Protein Information
5-oxoprolinase

UniProt Comments for OPLAH

OPLAH: Catalyzes the cleavage of 5-oxo-L-proline to form L- glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate. Defects in OPLAH are the cause of 5-oxoprolinase deficiency (OPLAHD). A disorder characterized by calcium oxalate/carbonate urolithiasis, and excessive urinary 5- oxo-L-proline. Affected individuals have recurrent episodes of vomiting, diarrhea, and abdominal pain. Belongs to the oxoprolinase family.

Protein type: Other Amino Acids Metabolism - glutathione; Hydrolase; EC 3.5.2.9

Molecular Function: 5-oxoprolinase (ATP-hydrolyzing) activity; hydrolase activity; nucleotide binding; catalytic activity; ATP binding

Biological Process: glutathione metabolic process
Show more

Research Articles on OPLAH

1. the cause of cellular ATP depletion in nephrotic cystinosis may be the futile cycle, formed between two ATP-dependant gamma-glutamyl cycle enzymes, gamma-glutamyl cysteine synthetase and 5-oxoprolinase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with OPLAH sirna

 Products by Pathway  Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Glutathione And One Carbon Metabolism Pathway antibodies  Glutathione And One Carbon Metabolism Pathway Diagram
 Glutathione Conjugation Pathway antibodies  Glutathione Conjugation Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Synthesis And Recycling Pathway antibodies  Glutathione Synthesis And Recycling Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Phase II Conjugation Pathway antibodies  Phase II Conjugation Pathway Diagram
 Gamma-glutamyl Cycle Pathway antibodies  Gamma-glutamyl Cycle Pathway Diagram

Organs/Tissues associated with OPLAH sirna

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1 publications with OPLAH and Brain
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