TIMM13 siRNA – Mitochondrial Import Inner Membrane Translocase Subunit Tim13 (Mouse)

Name: TIMM13 siRNA; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8241341
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8241341

MBS8241341 is an siRNA targeting TIMM13. Also known as TIMM13 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit TIMM13 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TIMM13, siRNA

Full Product Name

TIMM13 siRNA (Mouse)

Product Gene Name

TIMM13 sirna

Product Synonym Names

TIM13A; TIMM13A; Mitochondrial import inner membrane translocase subunit Tim13

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

TIMM13 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

10,458 Da

3D Structure

ModBase 3D Structure for P62075

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TIMM13 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for TIMM13 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of TIMM13 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TIMM13. It may not necessarily be applicable to this product.

NCBI GI #

7305581

NCBI GeneID

30055

NCBI Accession #

NP_038923.1

UniProt Primary Accession #

P62075

UniProt Secondary Accession #

Q91VM6; Q9DC89; Q9UHL8; Q9WTL1

UniProt Related Accession #

P62075

GenBank Nucleotide #

NM_013895.4

NCBI Official Full Name

mitochondrial import inner membrane translocase subunit Tim13

NCBI Official Synonym Full Names

translocase of inner mitochondrial membrane 13

NCBI Official Symbol

Timm13

NCBI Official Synonym Symbols

Tim9; Timm9; D10Ertd378e

UniProt Protein Name

Mitochondrial import inner membrane translocase subunit Tim13

UniProt Gene Name

Timm13

UniProt Synonym Gene Names

Tim13a; Timm13a

UniProt Entry Name

TIM13_MOUSE

NCBI Protein Information

mitochondrial import inner membrane translocase subunit Tim13

UniProt Comments for TIMM13

TIMM13: Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins. Belongs to the small Tim family.

Protein type: Mitochondrial

Cellular Component: membrane; mitochondrion; mitochondrial inner membrane; nucleolus

Molecular Function: metal ion binding

Biological Process: protein transport; transport

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TIMM13 sirna

Reference Product	PubMed Publications
Timm23 sirna	>3 publications with TIMM13 and Timm23

Pathways associated with TIMM13 sirna

Products by Pathway	Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Mitochondrial Protein Import Pathway antibodies	Mitochondrial Protein Import Pathway Diagram

Diseases associated with TIMM13 sirna

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>3 publications with TIMM13 and Nervous System Diseases
Abnormalities, Multiple Antibodies	>1 publications with TIMM13 and Abnormalities, Multiple

Organs/Tissues associated with TIMM13 sirna

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>2 publications with TIMM13 and Liver
Brain Antibodies	>2 publications with TIMM13 and Brain
Skin Antibodies	>1 publications with TIMM13 and Skin
Muscle Antibodies	>1 publications with TIMM13 and Muscle
Kidney Antibodies	>1 publications with TIMM13 and Kidney