USP45 siRNA – Ubiquitin Carboxyl-Terminal Hydrolase 45 Isoform 2 (Mouse)

Name: USP45 siRNA; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8206872
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8206872

MBS8206872 is an siRNA targeting USP45. Also known as USP45 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit USP45 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

USP45, siRNA

Full Product Name

USP45 siRNA (Mouse)

Product Gene Name

USP45 sirna

Product Synonym Names

Ubiquitin carboxyl-terminal hydrolase 45; Deubiquitinating enzyme 45; Ubiquitin thioesterase 45; Ubiquitin-specific-processing protease 45

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

USP45 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

85,110 Da

3D Structure

ModBase 3D Structure for Q8K387

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse USP45 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for USP45 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of USP45 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for USP45. It may not necessarily be applicable to this product.

NCBI GI #

594150411

NCBI GeneID

77593

NCBI Accession #

NP_001277354.1

UniProt Primary Accession #

Q8K387

UniProt Secondary Accession #

Q8BU19; Q8BZ19; A2AJT1

UniProt Related Accession #

Q8K387

GenBank Nucleotide #

NM_001290425.1

NCBI Official Full Name

ubiquitin carboxyl-terminal hydrolase 45 isoform 2

NCBI Official Synonym Full Names

ubiquitin specific petidase 45

NCBI Official Symbol

Usp45

NCBI Official Synonym Symbols

Gcap7; AI843191; 3110003C05Rik; 4930550B20Rik

UniProt Protein Name

Ubiquitin carboxyl-terminal hydrolase 45

UniProt Synonym Protein Names

Deubiquitinating enzyme 45; Ubiquitin thioesterase 45; Ubiquitin-specific-processing protease 45

Protein Family

Secreted 45 kDa protein

UniProt Gene Name

Usp45

UniProt Entry Name

UBP45_MOUSE

NCBI Protein Information

ubiquitin carboxyl-terminal hydrolase 45

UniProt Comments for USP45

USP45: Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-specific protease; Protease; EC 3.4.19.12; Ubiquitin conjugating system

Molecular Function: peptidase activity; hydrolase activity; zinc ion binding; metal ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; cysteine-type peptidase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; proteolysis

Research Articles on USP45

1. these results establish USP45 as a new regulator of XPF-ERCC1 crucial for efficient DNA repair

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with USP45 sirna

Reference Product	PubMed Publications
Asb2 sirna	>1 publications with USP45 and Asb2
Eif3e sirna	>1 publications with USP45 and Eif3e

Diseases associated with USP45 sirna

Disease Name	Pubmed Publications
Brain Diseases Antibodies	>1 publications with USP45 and Brain Diseases
Death Antibodies	>1 publications with USP45 and Death
Nervous System Diseases Antibodies	>1 publications with USP45 and Nervous System Diseases
Disease Models, Animal Antibodies	>1 publications with USP45 and Disease Models, Animal
Necrosis Antibodies	>1 publications with USP45 and Necrosis

Organs/Tissues associated with USP45 sirna

Organ/Tissue Name	Pubmed Publications
Other Antibodies	>12 publications with USP45 and Other
Intestine Antibodies	>4 publications with USP45 and Intestine
Eye Antibodies	>1 publications with USP45 and Eye
Pancreas Antibodies	>1 publications with USP45 and Pancreas
Liver Antibodies	>1 publications with USP45 and Liver