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HSD17B12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6308484

MBS6308484 is an antibody targeting HSD17B12. Also known as HSD17B12, ID (HSD17B12, Estradiol 17-beta-dehydrogenase 12, 17-beta-hydroxysteroid dehydrogenase 12, 3-ketoacyl-CoA reductase) (PE). Reactive in Human. Host: Rabbit. More details

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Product Overview

Online Datasheet in PDF
Product Name
HSD17B12, Polyclonal Antibody
Full Product Name
HSD17B12, ID (HSD17B12, Estradiol 17-beta-dehydrogenase 12, 17-beta-hydroxysteroid dehydrogenase 12, 3-ketoacyl-CoA reductase) (PE)
Product Gene Name
anti-HSD17B12 antibody
Product Synonym Names
HSD17B12; Estradiol 17-beta-dehydrogenase 12; 17-beta-hydroxysteroid dehydrogenase 12; 3-ketoacyl-CoA reductase
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human HSD17B12.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
34,324 Da
OMIM
609574
3D Structure
ModBase 3D Structure for Q53GQ0
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 126-155 in the central region of human HSD17B12, UniProt Accession #Q53GQ0. Species sequence homology: monkey
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-HSD17B12 antibody

Antibodies; Enzymes; Dehydrogenase
Applications Tested/Suitable for anti-HSD17B12 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
Application Notes for anti-HSD17B12 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-HSD17B12 antibody

Western Blot analysis of A2058 cell line lysates (35ug/lane) usingMBS6013293. HSD17B12 (arrow) was detected usingMBS6013293.
Rabbit Anti-HSD17B12 Polyclonal Antibody – Cat# MBS6308484

Immunohistochemistry (IHC) of anti-HSD17B12 antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma usingMBS6013293, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.
Rabbit Anti-HSD17B12 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6308484

Flow Cytometry (FC/FACS) of anti-HSD17B12 antibody

Flow Cytometric analysis of A2058 cells using MBS6013293 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-HSD17B12 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6308484

Product References and Citations for anti-HSD17B12 antibody

Plourde, M. J. Steroid Biochem. Mol. Biol. 116 (3-5), 134-153 (2009). Nagasaki, S. Mol. Cell. Endocrinol. 307 (1-2), 163-168 (2009). Bellemare, V. J. Steroid Biochem. Mol. Biol. 114 (3-5), 129-134 (2009).

Other Notes

Small volumes of anti-HSD17B12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HSD17B12. It may not necessarily be applicable to this product.

NCBI GI #
7705855
NCBI GeneID
51144
NCBI Accession #
NP_057226.1
UniProt Primary Accession #
Q53GQ0
UniProt Secondary Accession #
Q96JU2; Q9Y6G8; A8K9B0; D3DR23
UniProt Related Accession #
Q53GQ0
GenBank Nucleotide #
NM_016142.2
NCBI Official Full Name
estradiol 17-beta-dehydrogenase 12
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 12
NCBI Official Symbol
HSD17B12
NCBI Official Synonym Symbols
KAR; SDR12C1
UniProt Protein Name
Estradiol 17-beta-dehydrogenase 12
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase 12; 17-beta-HSD 12; 3-ketoacyl-CoA reductase
Protein Family
Very-long-chain 3-oxoacyl-CoA reductase
UniProt Gene Name
HSD17B12
UniProt Synonym Gene Names
17-beta-HSD 12; KAR
UniProt Entry Name
DHB12_HUMAN
NCBI Protein Information
estradiol 17-beta-dehydrogenase 12; 17-beta-HSD 12; 17beta-HSD type 12; 3-ketoacyl-CoA reductase; steroid dehydrogenase homolog; 17-beta-hydroxysteroid dehydrogenase 12; short chain dehydrogenase/reductase family 12C, member 1

NCBI Summary for HSD17B12

This gene encodes a very important 17beta-hydroxysteroid dehydrogenase (17beta-HSD) that converts estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. [provided by RefSeq, Oct 2011]

UniProt Comments for HSD17B12

HSD17B12: Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: Membrane protein, integral; Oxidoreductase; Lipid Metabolism - androgen and estrogen; EC 1.1.1.62; Lipid Metabolism - unsaturated fatty acid biosynthesis; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: extracellular matrix; endoplasmic reticulum membrane; integral to membrane

Molecular Function: heparin binding; collagen binding; estradiol 17-beta-dehydrogenase activity; protein binding; fibronectin binding

Biological Process: extracellular matrix organization and biogenesis; triacylglycerol biosynthetic process; cellular lipid metabolic process; estrogen biosynthetic process; fatty acid biosynthetic process
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Research Articles on HSD17B12

1. Significant associations have been found between CpG sites and patient sex, including DNA methylation in CASP6, a gene that may respond to estradiol treatment, and in HSD17B12, which encodes a sex steroid hormone.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-HSD17B12 antibody

 Products by Pathway  Pathway Diagram
 Androgen Biosynthesis Pathway antibodies  Androgen Biosynthesis Pathway Diagram
 Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies  Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
 Biosynthesis Of Unsaturated Fatty Acids Pathway antibodies  Biosynthesis Of Unsaturated Fatty Acids Pathway Diagram
 Fatty Acyl-CoA Biosynthesis Pathway antibodies  Fatty Acyl-CoA Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway antibodies  Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway Diagram
 Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway antibodies  Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway Diagram
 Fatty Acid Elongation Pathway antibodies  Fatty Acid Elongation Pathway Diagram
 Fatty Acid Elongation Pathway antibodies  Fatty Acid Elongation Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram
 Fatty Acid Metabolism Pathway antibodies  Fatty Acid Metabolism Pathway Diagram

Diseases associated with anti-HSD17B12 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >11 publications with HSD17B12 and Neoplasms
 Breast Neoplasms Antibodies  >4 publications with HSD17B12 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >3 publications with HSD17B12 and Neoplasms, Experimental
 Prostatic Neoplasms Antibodies  >3 publications with HSD17B12 and Prostatic Neoplasms
 Lung Diseases Antibodies  >2 publications with HSD17B12 and Lung Diseases
 Inflammation Antibodies  >2 publications with HSD17B12 and Inflammation
 Atrophy Antibodies  >1 publications with HSD17B12 and Atrophy
 Lung Neoplasms Antibodies  >1 publications with HSD17B12 and Lung Neoplasms
 Heart Diseases Antibodies  >1 publications with HSD17B12 and Heart Diseases
 Cardiovascular Diseases Antibodies  >1 publications with HSD17B12 and Cardiovascular Diseases

Organs/Tissues associated with anti-HSD17B12 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >9 publications with HSD17B12 and Blood
 Prostate Antibodies  >3 publications with HSD17B12 and Prostate
 Lung Antibodies  >2 publications with HSD17B12 and Lung
 Heart Antibodies  >1 publications with HSD17B12 and Heart
 Muscle Antibodies  >1 publications with HSD17B12 and Muscle
 Vascular Antibodies  >1 publications with HSD17B12 and Vascular
 Embryonic Tissue Antibodies  >1 publications with HSD17B12 and Embryonic Tissue
 Uncharacterized Tissue Antibodies  >1 publications with HSD17B12 and Uncharacterized Tissue
 Lymph Node Antibodies  >1 publications with HSD17B12 and Lymph Node
 Lymph Antibodies  >1 publications with HSD17B12 and Lymph
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