PCAF Antibody – Rabbit Polyclonal

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq, Jul 2008]

PCAF: Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Interacts with SIRT1. Interacts (unsumoylated form) with NR2C1; the interaction promotes transactivation activity. Interacts with EP300, CREBBP and DDX17. Interacts with NCOA1 and NCOA3. Component of a large chromatin remodeling complex, at least composed of MYSM1, KAT2B/PCAF, RBM10 and KIF11/TRIP5. Interacts with NR2C2 (hypophosphorylated and unsumoylated form); the interaction promotes the transactivation activity of NR2C2. Binds to HTLV-1 Tax. Interacts with and acetylates HIV-1 Tat. Interacts with KLF1; the interaction does not acetylate KLF1 and there is no enhancement of its transactivational activity. Interacts with NFE4. Interacts with MECOM. Interacts with E2F1; the interaction acetylates E2F1 augmenting its DNA-binding and transcriptional activity. Ubiquitously expressed but most abundant in heart and skeletal muscle. Belongs to the GCN5 family.

Protein type: Acetyltransferase; EC 2.3.1.48; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 3p24

Cellular Component: nucleoplasm; kinetochore; I band; PCAF complex; actomyosin; nucleus; A band

Molecular Function: cyclin-dependent protein kinase inhibitor activity; lysine N-acetyltransferase activity; protein binding; histone acetyltransferase activity; histone deacetylase binding; acetyltransferase activity; transcription coactivator activity; protein complex binding; transcription cofactor activity; transcription factor binding; protein kinase binding

Biological Process: transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; Notch signaling pathway; internal peptidyl-lysine acetylation; viral reproduction; rhythmic process; negative regulation of cyclin-dependent protein kinase activity; transcription from RNA polymerase I promoter; chromatin remodeling; negative regulation of cell proliferation; protein amino acid acetylation; cellular response to insulin stimulus; positive regulation of transcription from RNA polymerase II promoter; gene expression; cell cycle arrest; transcription initiation from RNA polymerase I promoter; peptidyl-lysine acetylation; N-terminal peptidyl-lysine acetylation