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vwf Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1759582

MBS1759582 is an antibody targeting vwf. Also known as Anti-Zebrafish vwf Antibody. Reactive in Zebrafish. Host: Rabbit. More details

$585 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
vwf, Polyclonal Antibody
Full Product Name
Anti-Zebrafish vwf Antibody
Product Gene Name
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Zebrafish
Form/Format
Liquid. Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Purity/Purification
Immunogen affinity purified.
Concentration
500ug/ml (lot specific)
Specificity
PredominantlyExpressed in adult forebrain.Expressed in fetal brain, lung and kidney.
Preparation and Storage
At -20 degree C for one year, at 4 degree C for one month. Avoid repeated freezing and thawing.
Subcellular Localization
Secreted
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Protein Function
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Immunohistochemistry (IHC) of anti-vwf antibody

Figure 1. IHC analysis of Vwf using anti-Vwf antibody (MBS1759582).
Vwf was detected in a paraffin-embedded section of Zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Vwf Antibody (MBS1759582) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-vwf Polyclonal Antibody – Cat# MBS1759582

Immunohistochemistry (IHC) of anti-vwf antibody

Figure 2. IHC analysis of Vwf using anti-Vwf antibody (MBS1759582).
Vwf was detected in a paraffin-embedded section of Zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Vwf Antibody (MBS1759582) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-vwf Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1759582

Immunohistochemistry (IHC) of anti-vwf antibody

Figure 3. IHC analysis of Vwf using anti-Vwf antibody (MBS1759582).
Vwf was detected in a paraffin-embedded section of Zebrafish gill tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Vwf Antibody (MBS1759582) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-vwf Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1759582

Immunohistochemistry (IHC) of anti-vwf antibody

Figure 4. IHC analysis of Vwf using anti-Vwf antibody (MBS1759582).
Vwf was detected in a paraffin-embedded section of Zebrafish gill tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Vwf Antibody (MBS1759582) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-vwf Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1759582

Other Notes

Small volumes of anti-vwf antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for vwf. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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