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GHRL siRNA – Appetite-Regulating Hormone Preproprotein (Rat)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8239145

MBS8239145 is an siRNA targeting GHRL. Also known as GHRL siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit GHRL expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GHRL, siRNA
Full Product Name
GHRL siRNA (Rat)
Product Gene Name
GHRL sirna
Product Synonym Names
Appetite-regulating hormone; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
GHRL siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
13,048 Da
3D Structure
ModBase 3D Structure for Q9QYH7
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GHRL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for GHRL sirna
RNA Interference (RNAi)

Other Notes

Small volumes of GHRL sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GHRL. It may not necessarily be applicable to this product.

NCBI GI #
11067387
NCBI GeneID
59301
NCBI Accession #
NP_067701.1
UniProt Primary Accession #
Q9QYH7
UniProt Secondary Accession #
Q9ET69
UniProt Related Accession #
Q9QYH7
GenBank Nucleotide #
NM_021669.2
NCBI Official Full Name
appetite-regulating hormone preproprotein
NCBI Official Synonym Full Names
ghrelin/obestatin prepropeptide
NCBI Official Symbol
Ghrl
UniProt Protein Name
Appetite-regulating hormone
UniProt Synonym Protein Names
Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide
Protein Family
Appetite-regulating hormone
UniProt Gene Name
Ghrl
UniProt Entry Name
GHRL_RAT
NCBI Protein Information
appetite-regulating hormone

NCBI Summary for GHRL

endogenous ligand for the growth hormone secretagogue receptor (GHSR); involved in testicular function and GH release [RGD, Feb 2006]

UniProt Comments for GHRL

ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach

Protein type: Secreted; Cell cycle regulation; Cell development/differentiation; Apoptosis; Secreted, signal peptide

Cellular Component: extracellular space; axon; cytoplasm; extracellular region

Molecular Function: growth hormone-releasing hormone activity; G-protein-coupled receptor binding; ghrelin receptor binding; hormone activity; protein tyrosine kinase activator activity

Biological Process: negative regulation of circadian sleep/wake cycle, REM sleep; positive regulation of cortisol secretion; hormone-mediated signaling; activation of MAPK activity; response to hormone stimulus; negative regulation of interleukin-6 biosynthetic process; decidualization; positive regulation of response to food; negative regulation of insulin secretion; positive regulation of adrenocorticotropic hormone secretion; elevation of cytosolic calcium ion concentration; negative regulation of locomotion; positive regulation of appetite; dendrite development; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of circadian sleep/wake cycle, non-REM sleep; regulation of response to food; gastric acid secretion; positive regulation of insulin secretion; adult feeding behavior; negative regulation of interleukin-1 beta production; regulation of cell proliferation; positive regulation of growth hormone secretion; G-protein coupled receptor protein signaling pathway; positive regulation of synaptogenesis; response to estrogen stimulus; negative regulation of inflammatory response; actin polymerization and/or depolymerization; negative regulation of endothelial cell proliferation; regulation of excitatory postsynaptic membrane potential; negative regulation of apoptosis
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Research Articles on GHRL

1. The data showed that intraportal infusion of ghrelin exerted an inhibitory effect on GLP-1 secretion through growth hormone secretagogue receptor 1alpha (GHS1alpha receptor).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GHRL sirna

 Reference Product  PubMed Publications
 Ghsr sirna  >30 publications with GHRL and Ghsr
 Lep sirna  >12 publications with GHRL and Lep
 Cck sirna  >8 publications with GHRL and Cck
 Gcg sirna  >3 publications with GHRL and Gcg
 Igf1 sirna  >1 publications with GHRL and Igf1
 Mboat4 sirna  >1 publications with GHRL and Mboat4
 Ins1 sirna  >1 publications with GHRL and Ins1

Pathways associated with GHRL sirna

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram

Diseases associated with GHRL sirna

 Disease Name  Pubmed Publications
 Obesity Antibodies  >56 publications with GHRL and Obesity
 Insulin Resistance Antibodies  >18 publications with GHRL and Insulin Resistance
 Inflammation Antibodies  >12 publications with GHRL and Inflammation
 Weight Gain Antibodies  >12 publications with GHRL and Weight Gain
 Cachexia Antibodies  >8 publications with GHRL and Cachexia
 Breast Neoplasms Antibodies  >7 publications with GHRL and Breast Neoplasms
 Kidney Diseases Antibodies  >5 publications with GHRL and Kidney Diseases
 Heart Diseases Antibodies  >5 publications with GHRL and Heart Diseases
 Necrosis Antibodies  >5 publications with GHRL and Necrosis
 Hypertension Antibodies  >4 publications with GHRL and Hypertension
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