OSGEP siRNA – Probable Trna N6-Adenosine Threonylcarbamoyltransferase (Rat)

Name: OSGEP siRNA; Rat; MyBioSource
Brand: MyBioSource
SKU: MBS827407
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS827407

MBS827407 is an siRNA targeting OSGEP. Also known as OSGEP siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit OSGEP expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

OSGEP, siRNA

Full Product Name

OSGEP siRNA (Rat)

Product Gene Name

OSGEP sirna

Product Synonym Names

GCPL1; Probable tRNA N6-adenosine threonylcarbamoyltransferase; N6-L-threonylcarbamoyladenine synthase; t(6)A synthase; O-sialoglycoprotein endopeptidase; t(6)A37 threonylcarbamoyladenosine biosynthesis protein Osgep; tRNA threonylcarbamoyladenosine biosynthesis protein Osgep

Host

Synthetic

Species Reactivity

Rat

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

OSGEP siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

36,357 Da

3D Structure

ModBase 3D Structure for Q9WVS2

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat OSGEP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for OSGEP sirna

RNA Interference (RNAi)

Other Notes

Small volumes of OSGEP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OSGEP. It may not necessarily be applicable to this product.

NCBI GI #

189303591

NCBI GeneID

290028

NCBI Accession #

NP_001093980.1

UniProt Primary Accession #

Q9WVS2

UniProt Secondary Accession #

B0BMW7

UniProt Related Accession #

Q9WVS2

GenBank Nucleotide #

NM_001100510.1

NCBI Official Full Name

probable tRNA N6-adenosine threonylcarbamoyltransferase

NCBI Official Synonym Full Names

O-sialoglycoprotein endopeptidase

NCBI Official Symbol

Osgep

UniProt Protein Name

Probable tRNA N6-adenosine threonylcarbamoyltransferase

UniProt Synonym Protein Names

N6-L-threonylcarbamoyladenine synthase; t(6)A synthase; O-sialoglycoprotein endopeptidase

Protein Family

Probable tRNA N6-adenosine threonylcarbamoyltransferase

UniProt Gene Name

Osgep

UniProt Synonym Gene Names

Gcpl1; t(6)A synthase

UniProt Entry Name

OSGEP_RAT

NCBI Protein Information

probable tRNA N6-adenosine threonylcarbamoyltransferase

UniProt Comments for OSGEP

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. OSGEP likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with OSGEP sirna

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>1 publications with OSGEP and Heart