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SLC8A1 siRNA – Sodium/Calcium Exchanger 1 Isoform 2 (Rat)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8223374

MBS8223374 is an siRNA targeting SLC8A1. Also known as SLC8A1 siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit SLC8A1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SLC8A1, siRNA
Full Product Name
SLC8A1 siRNA (Rat)
Product Gene Name
SLC8A1 sirna
Product Synonym Names
NCX1; Sodium/calcium exchanger 1; Na(+)/Ca(2+)-exchange protein 1; Solute carrier family 8 member 1
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
SLC8A1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
108,126 Da
3D Structure
ModBase 3D Structure for Q01728
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SLC8A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for SLC8A1 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of SLC8A1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC8A1. It may not necessarily be applicable to this product.

NCBI GI #
398650632
NCBI GeneID
29715
NCBI Accession #
NP_001257701.1
UniProt Primary Accession #
Q01728
UniProt Secondary Accession #
Q924Y2; Q9QW49; Q9R238; Q9R239; Q9WU29; Q9WU30
UniProt Related Accession #
Q01728
GenBank Nucleotide #
NM_001270772.1
NCBI Official Full Name
sodium/calcium exchanger 1 isoform 2
NCBI Official Synonym Full Names
solute carrier family 8 (sodium/calcium exchanger), member 1
NCBI Official Symbol
Slc8a1
NCBI Official Synonym Symbols
Ncx; Ncx1
UniProt Protein Name
Sodium/calcium exchanger 1
UniProt Synonym Protein Names
Na(+)/Ca(2+)-exchange protein 1; Solute carrier family 8 member 1
Protein Family
Sodium/calcium exchanger
UniProt Gene Name
Slc8a1
UniProt Synonym Gene Names
Ncx1
UniProt Entry Name
NAC1_RAT
NCBI Protein Information
sodium/calcium exchanger 1

NCBI Summary for SLC8A1

displays Na+ gradient-dependent Ca2+ transport activity; plays a role in regulation of calcium ion transport [RGD, Feb 2006]

UniProt Comments for SLC8A1

NCX1: Rapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores. Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral; Transporter

Cellular Component: microtubule; mitochondrion; basolateral plasma membrane; integral to plasma membrane; cell; T-tubule; integral to membrane; dendritic spine; Z disc; cytosol; cell projection; membrane; plasma membrane; dendritic shaft; intracellular; sarcolemma

Molecular Function: calmodulin binding; protein binding; metal ion binding; cytoskeletal protein binding; ankyrin binding; calcium:sodium antiporter activity

Biological Process: heart morphogenesis; embryonic placenta development; sodium ion transport; vascular smooth muscle contraction; post-embryonic development; calcium ion homeostasis; elevation of cytosolic calcium ion concentration; cardiac muscle cell development; calcium ion transport; response to glucose stimulus; cell communication; muscle fiber development; response to nutrient; regulation of the force of heart contraction; cardiac muscle contraction; response to drug; cellular sodium ion homeostasis; regulation of heart rate; regulation of calcium ion transport; regulation of sodium ion transport; response to ATP; cellular calcium ion homeostasis; telencephalon development; response to hydrogen peroxide; reduction of cytosolic calcium ion concentration; response to hypoxia; embryonic heart tube development
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Research Articles on SLC8A1

1. identified miR-132 as a novel microRNA targeting the NCX1, whose expression increased during hypoxia

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SLC8A1 sirna

 Reference Product  PubMed Publications
 Atp2a2 sirna  >5 publications with SLC8A1 and Atp2a2
 Atp1a2 sirna  >1 publications with SLC8A1 and Atp1a2
 Prkg1 sirna  >1 publications with SLC8A1 and Prkg1

Pathways associated with SLC8A1 sirna

 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram

Diseases associated with SLC8A1 sirna

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >12 publications with SLC8A1 and Cardiovascular Diseases
 Heart Diseases Antibodies  >10 publications with SLC8A1 and Heart Diseases
 Heart Failure Antibodies  >4 publications with SLC8A1 and Heart Failure
 Arrhythmias, Cardiac Antibodies  >4 publications with SLC8A1 and Arrhythmias, Cardiac
 Hypertension Antibodies  >4 publications with SLC8A1 and Hypertension
 Disease Models, Animal Antibodies  >4 publications with SLC8A1 and Disease Models, Animal
 Kidney Diseases Antibodies  >2 publications with SLC8A1 and Kidney Diseases
 Tachycardia Antibodies  >2 publications with SLC8A1 and Tachycardia
 Tachycardia, Ventricular Antibodies  >2 publications with SLC8A1 and Tachycardia, Ventricular
 Ventricular Dysfunction, Left Antibodies  >2 publications with SLC8A1 and Ventricular Dysfunction, Left

Organs/Tissues associated with SLC8A1 sirna

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >3 publications with SLC8A1 and Kidney
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