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RECQL5 siRNA – ATP-Dependent DNA Helicase Q5 (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8203194

MBS8203194 is an siRNA targeting RECQL5. Also known as RECQL5 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit RECQL5 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RECQL5, siRNA
Full Product Name
RECQL5 siRNA (Mouse)
Product Gene Name
Product Synonym Names
ATP-dependent DNA helicase Q5; DNA helicase. RecQ-like type 5; RecQ5; RECQL5beta; RecQ protein-like 5
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
RECQL5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
108,246 Da
3D Structure
ModBase 3D Structure for Q8VID5
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RECQL5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for RECQL5 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of RECQL5 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RECQL5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ATP-dependent DNA helicase Q5
NCBI Official Synonym Full Names
RecQ protein-like 5
NCBI Official Symbol
Recql5
NCBI Official Synonym Symbols
RecQ5; Recq5b; Recql5b
UniProt Protein Name
ATP-dependent DNA helicase Q5
UniProt Synonym Protein Names
DNA helicase, RecQ-like type 5; RecQ5; RECQL5beta; RecQ protein-like 5
UniProt Gene Name
Recql5
UniProt Synonym Gene Names
RecQ5
UniProt Entry Name
RECQ5_MOUSE
NCBI Protein Information
ATP-dependent DNA helicase Q5

UniProt Comments for RECQL5

RECQL5: May have an important role in DNA metabolism. Interacts with TOP3A and TOP3B. Ubiquitous. Belongs to the helicase family. RecQ subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Helicase; DNA repair, damage; EC 3.6.4.12

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleolus; DNA-directed RNA polymerase II, holoenzyme; nucleus

Molecular Function: DNA helicase activity; nucleic acid binding; hydrolase activity; nucleotide binding; ATP-dependent helicase activity; helicase activity; ATP binding

Biological Process: mitosis; cell division; DNA replication; cell cycle; DNA repair; response to DNA damage stimulus; DNA duplex unwinding; chromosome separation; DNA recombination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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