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APRT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9201046

A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine… More details

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Product Overview

Online Datasheet in PDF
Product Name
APRT, Polyclonal Antibody
Full Product Name
APRT Antibody (N-term)
Product Gene Name
anti-APRT antibody
Product Synonym Names
Adenine phosphoribosyltransferase; APRT; APRT
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Specificity
This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human APRT.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
APRT peptide (MBS9224925) is used for blocking the activity of APRT antibody (MBS9201046)
Molecular Weight
19608
Immunogen Sequence Positions
12-40
OMIM
102600
3D Structure
ModBase 3D Structure for P07741
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN

Product Categories/Family for anti-APRT antibody

Crown Antibodies; Metabolism; Signal Transduction
Applications Tested/Suitable for anti-APRT antibody
ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-APRT antibody
WB~~1:1000

Western Blot (WB) of anti-APRT antibody

APRT Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the APRT antibody detected the APRT protein (arrow).
Rabbit Anti-APRT Polyclonal Antibody – Cat# MBS9201046

Immunofluorescence (IF) of anti-APRT antibody

Fluorescent image of A549 cell stained with APRT Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with APRT primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).APRT immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Rabbit Anti-APRT Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9201046

Immunohistochemistry (IHC) of anti-APRT antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-APRT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9201046

Flow Cytometry (FC/FACS) of anti-APRT antibody

Flow cytometric analysis of HepG2 cells using APRT Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-APRT Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS9201046

Product References and Citations for anti-APRT antibody

Silva,C.H., et. al.,J. Biomol. Struct. Dyn. 25 (6), 589-597 (2008)
Di Pietro,V., et. al., Clin. Biochem. 40 (1-2), 73-80 (2007)

Other Notes

Small volumes of anti-APRT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for APRT purchase

MBS9201046 is an antibody targeting APRT. Also known as APRT Antibody (N-term). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for APRT. It may not necessarily be applicable to this product.

NCBI GI #
4502171
NCBI GeneID
353
NCBI Accession #
NP_000476.1
NCBI Related Accession #
NP_001025189.1
UniProt Primary Accession #
P07741
UniProt Secondary Accession #
Q3KP55; Q68DF9; G5E9J2
UniProt Related Accession #
P07741
GenBank Nucleotide #
NM_000485.2
NCBI Official Full Name
adenine phosphoribosyltransferase isoform a
NCBI Official Synonym Full Names
adenine phosphoribosyltransferase
NCBI Official Symbol
APRT
NCBI Official Synonym Symbols
AMP; APRTD
UniProt Protein Name
Adenine phosphoribosyltransferase
Protein Family
Probable adenine phosphoribosyltransferase
UniProt Gene Name
APRT
UniProt Synonym Gene Names
APRT
UniProt Entry Name
APT_HUMAN
NCBI Protein Information
adenine phosphoribosyltransferase

NCBI Summary for APRT

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for APRT

APRT: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD); also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones. Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Protein type: Nucleotide Metabolism - purine; Transferase; EC 2.4.2.7

Chromosomal Location of Human Ortholog: 16q24

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: adenine binding; adenine phosphoribosyltransferase activity; AMP binding

Biological Process: lactation; grooming behavior; cellular response to insulin stimulus; nucleobase, nucleoside and nucleotide metabolic process; adenine salvage; purine salvage; purine base metabolic process

Disease: Adenine Phosphoribosyltransferase Deficiency
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Research Articles on APRT

1. A new mutation, p.Gln147X, in APRT gene, was found in a patient with adenine phosphoribosyltransferase deficiency.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-APRT antibody

 Reference Product  PubMed Publications
 HPRT1 antibody  >144 publications with APRT and HPRT1
 ADK antibody  >3 publications with APRT and ADK
 ADSL antibody  >2 publications with APRT and ADSL
 AMPD3 antibody  >1 publications with APRT and AMPD3
 ATIC antibody  >1 publications with APRT and ATIC

Pathways associated with anti-APRT antibody

 Products by Pathway  Pathway Diagram
 Polyketide Sugar Unit Biosynthesis Pathway antibodies  Polyketide Sugar Unit Biosynthesis Pathway Diagram
 Polyketide Sugar Unit Biosynthesis Pathway antibodies  Polyketide Sugar Unit Biosynthesis Pathway Diagram
 Polyketide Sugar Unit Biosynthesis Pathway antibodies  Polyketide Sugar Unit Biosynthesis Pathway Diagram
 Polyketide Sugar Unit Biosynthesis Pathway antibodies  Polyketide Sugar Unit Biosynthesis Pathway Diagram
 Polyketide Sugar Unit Biosynthesis Pathway antibodies  Polyketide Sugar Unit Biosynthesis Pathway Diagram
 Polyketide Sugar Unit Biosynthesis Pathway antibodies  Polyketide Sugar Unit Biosynthesis Pathway Diagram
 Polyketide Sugar Unit Biosynthesis Pathway antibodies  Polyketide Sugar Unit Biosynthesis Pathway Diagram
 Polyketide Sugar Unit Biosynthesis Pathway antibodies  Polyketide Sugar Unit Biosynthesis Pathway Diagram
 Polyketide Sugar Unit Biosynthesis Pathway antibodies  Polyketide Sugar Unit Biosynthesis Pathway Diagram

Diseases associated with anti-APRT antibody

 Disease Name  Pubmed Publications
 Urolithiasis Antibodies  >92 publications with APRT and Urolithiasis
 Kidney Diseases Antibodies  >51 publications with APRT and Kidney Diseases
 Carcinoma Antibodies  >28 publications with APRT and Carcinoma
 Disease Models, Animal Antibodies  >21 publications with APRT and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >8 publications with APRT and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >8 publications with APRT and Adenocarcinoma
 Heart Diseases Antibodies  >5 publications with APRT and Heart Diseases
 Fibrosis Antibodies  >4 publications with APRT and Fibrosis
 Acute Kidney Injury Antibodies  >4 publications with APRT and Acute Kidney Injury
 Inflammation Antibodies  >3 publications with APRT and Inflammation

Organs/Tissues associated with anti-APRT antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >121 publications with APRT and Blood
 Ovary Antibodies  >108 publications with APRT and Ovary
 Kidney Antibodies  >81 publications with APRT and Kidney
 Muscle Antibodies  >15 publications with APRT and Muscle
 Skin Antibodies  >13 publications with APRT and Skin
 Lung Antibodies  >12 publications with APRT and Lung
 Bone Antibodies  >12 publications with APRT and Bone
 Bone Marrow Antibodies  >10 publications with APRT and Bone Marrow
 Heart Antibodies  >9 publications with APRT and Heart
 Embryonic Tissue Antibodies  >7 publications with APRT and Embryonic Tissue
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